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重庆3个主要砂梨品种SUC基因克隆及表达分析

Clone and Expression Analyses of Sucrose Transporter Genes from 3 Main Varieties of Sand Pear in Chongqing
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摘要 蔗糖转运蛋白(sucrose/H^+ cotransporters或sucrose transporters,SUCs或SUTs)在调节同化产物的分配过程中具有重要作用。为研究梨果实发育成熟过程中关键的蔗糖转运蛋白及其生理功能,以重庆市主栽3个砂梨Pyrus pyrifolia品种"翠冠""黄花""圆黄"为试材,通过转录组测序获取高表达的SUC基因,随后采用进化聚类的方法对其生理功能进行预测。结果表明,在梨果实发育过程中高表达的SUC基因只有4个,命名为PrSUC1、PrSUC2、PrSUC3和PrSUC4;其中,PrSUC2、PrSUC3和PrSUC4在3个梨品种的果实中都有表达,而PrSUC1只在"黄花"果实中表达。进一步的qPCR分析表明,这4个基因在"黄花"的枝梢、叶片、花中同样都有较高的表达。进化聚类分析发现,PrSUC3和PrSUC4能够与拟南芥中Clade I/SUT1蛋白聚类,暗示它们更有可能参与了梨果实和其他组织中蔗糖的转运。
作者 王久照 谭嫣 马小迪 刘小刚 曾明 WANG Jiuzhao;TAN Yan;MA Xiaodi;LIU Xiaogang;ZENG Ming
出处 《中国南方果树》 北大核心 2019年第2期97-101,共5页 South China Fruits
基金 国家梨产业技术体系(CARS-28-41)重庆综合试验站资助
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