摘要
目的:检测Klotho和Beclin1蛋白在慢性宫颈炎、低级别鳞状上皮内病变(low grade squamous intraepithelial lesion,LSIL)、高级别鳞状上皮内病变(high grade squamous intraepithelial lesion,HSIL)和宫颈鳞状细胞癌(cervical squamous cell carcinoma,SCC)组织中的表达并探讨其临床意义。方法:采用免疫组织化学技术(Envision法)检测Klotho和Beclin1蛋白在各研究组中的表达情况,并分析以上两种蛋白表达情况与SCC病理特征的相关性,以及两种蛋白在SCC中表达的相关性。结果:Klotho蛋白在慢性宫颈炎组、LSIL组、HSIL组、SCC组中的阳性表达率分别为95%、92.86%、65.38%、27.90%, Beclin1蛋白在慢性宫颈炎组、LSIL组、HSIL组、SCC组中的阳性表达率分别为25.00%、28.57%、38.46%、74.42%,差异有统计学意义(P<0.05)。Klotho和Beclin1在SCC组织中的表达均与肿瘤分化程度有关(P<0.05),与患者年龄、肿瘤FIGO分期、淋巴结转移、肿块大小、浸润深度及脉管浸润无关(P>0.05)。在SCC组织中,Klotho和Beclin1的表达无相关性(P>0.05)。结论:Klotho与Beclin1的异常表达可能与宫颈鳞癌发生发展、浸润转移有关。
Objective:To detect the expression of Klotho and Beclin1 protein in chronic cervicitis,low grade squamous intraepithelial lesion(LSIL)group,high grade squamous intraepithelial lesion(HSIL)group and cervical squamous cell carcinoma(SCC)group.Methods:Immunohistochemical technique(Envision)was used to detect the expression of Klotho and Beclin1 proteins in each study group.The correlation between the expression of the above two proteins and the pathological features of SCC and the expression of the two proteins in SCC were analyzed.Results:The positive expression rates of Klotho protein in chronic cervicitis group,LSIL group,HSIL group and SCC group were 95%,92.86%,65.38%,27.90%,respectively.The positive rates of Beclin1 protein in chronic cervicitis group,LSIL group,HSIL group were 25.0%,28.57%,38.46%and 74.42%,respectively.The differences were statistically significant(P<0.05).The expression of Klotho and Beclin1 in SCC was correlated with the degree of tumor differentiation(P<0.05),but not with the age,figo stage,lymph node metastasis,tumor size,depth of invasion and vessel invasion(P>0.05).There was no correlation between the expression of Klotho and Beclin1 in scc tissues.Conclusion:The abnormal expression of klotho and beclin1 may be related to the development,invasion and metastasis of cervical squamous cell carcinoma.
作者
何蔚
任黔川
HE Wei;REN Qian-chuan(Department of Gynecology,the First Affiliated Hospital of Southwest Medical University,Luzhou,Sichuan Province,646000,China)
出处
《海南医学院学报》
CAS
2019年第8期572-577,共6页
Journal of Hainan Medical University
基金
泸州市科技计划项目2011-I-S36(5/5)~~
