摘要
试验选取厚朴酚、黄藤素、秦皮、大黄素和黄柏5种中药提取物,采用药敏纸片法和布氏肉汤稀释法对禽多杀性巴氏杆菌C48-1进行抑菌试验。体外抑菌试验结果表明,厚朴酚、黄藤素和秦皮提取物的抑菌圈大小分别为22.0、11.3、15.6 mm,最小抑菌浓度(MIC)分别为8、256、64μg/mL,最小杀菌浓度(MBC)分别为16、512、256μg/mL;大黄素和黄柏提取物对C48-1没有抑菌作用;厚朴酚的抑菌作用最强,秦皮次之,黄藤素最弱。培养禽多杀性巴氏杆菌C48-1时,培养基中加入厚朴酚含量为6μg/mL时,细菌的生长速度相比对照组受到明显抑制,当药物浓度为MIC(8μg/mL)值时,禽多杀性巴氏杆菌C48-1生长被完全抑制。从体内抑菌试验结果可知,厚朴酚对禽多杀性巴氏杆菌C48-1侵染江汉鸡的保护力最高,为50%。该结果为进一步研制中药制剂预防禽多杀性巴氏杆菌病提供了理论基础。
The experiment was conducted to explore the inhibition effect of magnolol,palmatine,cortex fraxini,emodin and cortex phellodendri on avian Pasteurella multocida C48-1 determined by disk diffusion method and double dilution method. The results of vitro antibacterial effect indicated that the diameter of inhibition zone of magnolol,palmatine,cortex fraxini were 22.0,11.3,15.6 mm,and the MIC were 8,256,64 μg/mL,and the MBC were 16,512,256 μg/mL,respectively. Emodin and cortex phellodendri had no antibacterial effect on P.multocida C48-1. Magnolol had the strongest antibacterial effect, followed by cortex fraxini,and palmatine had the weakest antibacterial effect on P.multocida C48-1. When P.multocida C48-1 were cultured in the content that magnolol was 6 μg/mL,the growth rate was significantly inhibited compared with the control group.P.multocida C48-1 won’t be completely inhibited until the magnolol’s content reached 8 μg/mL. According to the results of vivo antibacterial effect, the protective rate of magnolol was up to 50% when Jianghan chickens was infected by P. multocida C48-1. This study also provides an important basis for further studying to protect avian P. multocida disease.
作者
蔡萌
张文婷
汪最
汪琛
邵华斌
罗青平
江涛
CAI Meng;ZHANG Wen-ting;WANG Zui;WANG Chen;SHAO Hua-bin;LUO Qing-ping;JIANG Tao(College of Animal Sciences,Yangtze University,Jingzhou 434025,Hubei,China;Institute of Animal Husbandry and Veterinary,Hubei Academy of Agricultural Sciences,Wuhan 430064,China;Key Laboratory of Animal Disease Control and Prevention Formulation of Ministry of Agriculture,Wuhan 430064,China)
出处
《湖北农业科学》
2019年第7期89-91,126,共4页
Hubei Agricultural Sciences
基金
国家重点研发计划项目(2018YFD0500503)
国家肉鸡产业技术体系项目(CARS-11-G43)
关键词
中药提取物
禽多杀性巴氏杆菌
抑菌作用
extracts of traditional Chinese medicine
avian Pasteurella multocida
antibacterial effect
