摘要
灵芝具有重要的药用价值,培养条件的优化可提高灵芝中各种活性成分的含量。探讨了较高温度对灵芝发酵过程中的生理指标及灵芝酸产量的影响。结果表明,较高的发酵温度虽然降低了灵芝生物量,但提高了淀粉酶﹑漆酶﹑纤维素酶的酶活。发酵第4天,36℃培养条件下淀粉酶酶活达到最大值,比30℃发酵条件下的酶活提高了22.61%。发酵第10天,34℃培养条件下的漆酶酶活﹑纤维素酶酶活﹑可溶性糖含量和灵芝酸产量都达到最大值,相对于30℃的培养条件,分别提高了51.12%、30.41%、13.21%和45.23%。结果表明较高温度发酵有利于酶活和灵芝酸含量的提高,该研究为灵芝的高温发酵提供了新的思路和理论指导。
Ganoderma lucidum has important medicinal value, and the content of active ingredients in G. lucidum can be improved by optimizing the culture condition. The effect of higher temperature on the physiological indexes and the ganoderic acid yield in G. lucidum fermentation process were studied in this study. The results showed that though biomass of G. lucidum decreased, yet the enzyme activity of amylase, laccase and cellulase increased under the higher temperature. On day 4, the amylase activity reached the maximum at 36 ℃, and the enzyme activity increased by 22.61% higher than that under the fermentation condition at 30 ℃. On day 10, the laccase activity, cellulase activity, soluble sugar content and ganoderic acid yield reached the maximum value at 34 ℃, respectively increased by 51.12%, 30.41%, 13.21%, and 45.23% as compared with the fermentation temperature at 30 ℃. The results showed that higher fermentation temperature conducive to the increment of enzyme activity and improve ganoderic acid yield. The study provides new idea and theoretical guidance for higher temperature fermentation of G. lucidum.
作者
刘月芹
贺晓龙
任桂梅
LIU Yue-qin;HE Xiao-long;REN Gui-mei(Coll. of Life Sci., Yan′an Uni., Yanan 716000)
出处
《微生物学杂志》
CAS
CSCD
2019年第2期90-96,共7页
Journal of Microbiology
基金
博士科学研究启动项目(YDBK2015-08)
陕西省教育厅项目(202110029)
延安大学产学研合作项目(205110012)
延安大学校级项目(YDQ2016-46)
关键词
温度
灵芝
酶活
可溶性糖
灵芝酸
emperature
Ganoderma lucidum
enzyme activity
soluble sugar
ganoderic acid
