摘要
建立高效液相色谱-飞行时间质谱联用在线筛选并鉴定牛蒡提取物中抗氧化活性成分的方法。样品经在线色谱柱分离后进行分流,一路与稳定的1,1-二苯基-2-三硝基苯肼-甲醇溶液混合,实现在线筛选自由基清除剂;另一路进入二极管阵列检测器-串联三重四极杆飞行时间质谱,用于化合物的快速鉴别。负离子模式下,通过高分辨率质谱获得的相对分子质量结合化合物的紫外吸收特征,参考相关文献和部分对照品验证,从牛蒡乙酸乙酯相中筛选并鉴定出咖啡酸及衍生物19种。本方法可实现快速定向筛选和鉴定食品及中药等复杂体系中的抗氧化活性成分。
A new method based on high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-DAD-Q-TOF-MS) was developed for the rapid screening for and identification of antioxidant compounds in burdock root extract. The sample was divided into two parts by the first HPLC;one part flowed through the second HPLC with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution for on-line screening for the antioxidants;the other was flowed through DAD-Q-TOF-MS for on-line rapid identification. Caffeic acid and its 18 derivatives were identified by their relative molecular masses as determined by high resolution MS in the negative ion mode and by comparison of their UV absorption characteristics with literature data and reference standards. Overall, this method is a powerful tool for rapid screening for and identification of antioxidant compounds from complicated systems such as foods and Chinese traditional medicinal materials.
作者
郑振佳
张瑞凌
张敏敏
邱志常
张斌
乔旭光
ZHENG Zhenjia;ZHANG Ruiling;ZHANG Minmin;QIU Zhichang;ZHANG Bin;QIAO Xuguang(Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, China;Shandong Analysis and Test Center, Key Laboratory of Traditional Chinese Medicine Quality Control Technology, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第8期175-179,共5页
Food Science
基金
公益性行业(农业)科研专项(201503142)
国家自然科学基金青年科学基金项目(21506119)
郭兰萍泰山学者岗位平台
山东省2017年度农业重大应用技术创新项目
