摘要
目的观察肾衰Ⅱ号方对5/6(ablation/infarction,A/I)肾切除慢性肾衰竭(CRF)大鼠残余肾组织凋亡相关蛋白表达的影响。方法 SD雄性大鼠采用5/6(A/I)肾切除术制备CRF大鼠模型,随机分为模型组、肾衰Ⅱ号方组、西药组,每组15只,另取15只大鼠作为假手术组。各组给予相应干预,60 d后Western blot检测凋亡相关蛋白p53、Bax、Cleaved-parp的表达,免疫组化检测p53、Bax蛋白的表达,透射电镜观察肾组织超微结构。结果与假手术组比较,模型组大鼠肾皮质和肾髓质p53、Bax、Cleaved-parp蛋白表达明显升高(P<0.05);与模型组比较,各给药组大鼠肾皮质和肾髓质p53、Bax、Cleaved-parp蛋白表达明显降低(P<0.05)。免疫组化结果显示,p53、Bax蛋白表达主要位于肾小管,模型组大鼠炎性细胞浸润明显,蛋白表达较假手术组明显升高,各给药组较模型组炎性细胞浸润减轻,p53和Bax蛋白表达降低。透射电镜结果显示,假手术组大鼠肾皮质和肾髓质未见细胞凋亡,超微结构正常,模型组大鼠核固缩,细胞凋亡出现,超微结构紊乱,各给药组较模型组超微结构改善,少见细胞凋亡发生。结论肾衰Ⅱ号方可抑制5/6(A/I)肾切除CRF大鼠细胞凋亡,其作用机制可能与下调p53、Bax、Cleaved-parp蛋白表达相关。
Objective To observe the effects of ShenshuaiⅡPrescription(SSP)on the apoptosis-related protein expressions in the chronic renal failure(CRF)rats induced by 5/6(ablation/infarction,A/I)nephrectomy.Methods SD male rats were induced CRF rat model by 5/6(A/I)nephrectomy and were randomized into model group,SSP group and Western medicine group,with 15 rats in each group.Another 15 rats were recruited as sham-operation group.After 60 days of intervention,the apoptosis-related protein expressions of p53,Bax and Cleaved-parp were tested by Western blot,the protein expressions of p53 and Bax were tested by immunohistochemistry and renal tissue ultrastructure was observed by transmission electron microscope.Results Compared with sham-operation group,the expressions of p53,Bax,and Cleaved-parp in the renal cortex and the medulla increased significantly in model group(P<0.05).Compared with the model group,the protein expressions of p53,Bax and Cleaved-parp in renal cortex and renal medulla significantly decreased(P<0.05).The results of immunohistochemistry showed that the protein expressions of p53 and Bax were mainly located in renal tubules.The inflammatory cell infiltration was obvious in the model group,and the protein expressions were significantly higher than those in the sham-operation group.The inflammatory cell infiltration was reduced in the medicine-administered groups compared with the model group,and the protein expressions of p53 and Bax decreased.Transmission electron microscopy showed that there was no apoptosis in the renal cortex and renal medulla of the sham-operation group,the ultrastructure was normal,the model group was pyknosis,apoptosis occurred,and the ultrastructural disorder was disordered.The ultrastructure of each medicine-administered group was improved compared with the model group,and apoptosis was rare.Conclusions SSP can inhibit apoptosis in CRF rats induced by 5/6(A/I)nephrectomy,which can be associated with down-regulation of protein expressions of p53,Bax,and Cleaved-parp.
作者
王蒙
杨柳易
杨婧
王琛
WANG Meng;YANG Liuyi;YANG Jing;WANG Chen(Shanghai Key Laboratory of Traditional Chinese Clinical Medicine,Shanghai 200021,China)
出处
《中国中医药信息杂志》
CAS
CSCD
2019年第5期64-68,共5页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家自然科学基金(81573946)
上海市科学技术委员会科研计划项目(16401931700)
上海市进一步加快中医药事业发展三年行动计划建设项目(Y2018-2020-FWTX-7005)
关键词
肾衰Ⅱ号方
慢性肾衰竭
肾间质纤维化
肾功能
凋亡
大鼠
Shenshuai Ⅱ Prescription
chronic renal failure
renal interstitial fibrosis
renal function
apoptosis
rats
