摘要
目的提高炎宁糖浆的质量标准,有效控制制剂质量。方法采用薄层色谱法对炎宁糖浆中白花蛇舌草、鹿茸草进行定性鉴别,采用HPLC测定去乙酰车叶草酸甲酯的含量,建立HPLC指纹图谱分析方法。结果薄层色谱鉴别分离度高、专属性强、重现性好;含量测定指标去乙酰车叶草酸甲酯在0.08～0.8μg范围内呈良好的线性关系(r=1.000),平均回收率为104.68%,精密度RSD=0.89%,重复性RSD=0.55%;经采用《中药色谱指纹图谱相似度评价系统》对指纹图谱进行分析,标定了33个共有峰,确认4个峰分别为原儿茶酸、去乙酰车叶草酸甲酯、香草酸、咖啡酸,对10批炎宁糖浆进行相似度评价,其相似度均高于0.85。结论本方法简单准确、专属性强,可为提高炎宁糖浆质量标准提供依据。
Objective To improve the quality standard of Yanning Syrup and control the qulity of preparation.Methods The thin-layer chromatography method was used to conduct qualitative identification for Hedyotis diffiisa and Monochasma sheareri in Yanning Syrup.HPLC was used to measure the content of deacetyl asperulosidic acid methyl ester.The HPLC fingerprint analysis method was established.Results The method of the thin layer chromatographic condition was good enough.And the method was special and reproducible.The linear of deacetyl asperulosidic acid methyl ester was in the range of 0.08-0.8μg(r=1.000).The average recovery rate was 104.68%,RSD of precision was 0.89%,RSD of reproducibility was 0.55%.Besides,Similarity Evaluation System for Chromatographic Fingerprints of TCM was used to analyze and compare the fingerprints.Totally 33 common peaks were determined and four were calibrated including protocatechuic acid,deacetyl asperulosidic acid methyl ester,vanillic acid and caffeic acid.The similarity were all over 0.85 for 10 batches of Yanning Syrup.Conclusion The method is simple,accurate and specific,which can provide a reasonable basis for the improvement of quality standard of Yanning Syrup.
作者
周楠
武佳丽
曲范娜
张清波
孟云阁
孙小萌
笔雪艳
ZHOU Nan;WU Jiali;QU Fanna;ZHANG Qingbo;MENG Yunge;SUN Xiaomeng;BI Xueyan(Heilongjiang University of Chinese Medicine,Harbin 150040,China;Heilongjiang Institute for Food and Drug Control,Harbin 150088,China;Heilongjiang Food and Drug Administration,Harbin 150001,China)
出处
《中国中医药信息杂志》
CAS
CSCD
2019年第5期83-87,共5页
Chinese Journal of Information on Traditional Chinese Medicine
基金
<中国药典>药品标准提高(553)
