摘要
建立了超高效液相色谱-质谱/质谱法(UPLC-MS/MS)测定豆芽中多种植物生长调节剂残留量的方法。样品用酸化乙腈提取,浓缩置换试剂,经0. 2μm滤膜过滤,以C18色谱柱分离待测物,采用多反应监测(MRM)离子扫描模式,外标法进行定量,线性良好。21种植物生长调节剂的方法检出限为0. 001～0. 005 mg/kg,样品添加回收率为61. 5%～118. 3%,相对标准偏差为0. 6%～8. 3%(n=6)。该方法简单快捷,定量准确,可满足豆芽中21种植物生长调节剂的残留量检测要求。
An effective method of liquid chromatography-mass spectrometry/mass spectrometry (UPLC-MS/MS) was established to detect the residues of various plant growth regulators in bean sprouts.The samples were initially extracted with acetonitrile and concentrated,which were filtered by 0.2 jim filter membrane afterwards.Then the eluate was separated by gradient elution with methanol-water solution of different ratio on C18(100mm×2.1mm×1.7μm) column.It was detected under the mode of positive multiple reactions monitoring(MRM).The external standard method was adopted for quantitative analysis,the correlation coefficients was more than 0.990.Finally,the detection limit of 21 types of plant growth regulators,for all the samples,ranged from 0.001 to 0.005 mg/kg,the recoveries of samples were 61.5% to 118.3% and the relative standard deviations were in the range of 0.6% to 8.3%( n = 6).In conclusion,it was a simple and efficient method which can meet the requirements for residue testing of 21 types of plant growth regulators in bean sprouts.
作者
黄志波
何健安
梁志刚
何少斌
李艳芳
HUANG Zhi-bo;HE Jian-an;LIANG Zhi-gang;HE Shao-bin;LI Yan-fang(Dongguan Supervision and Test Institution of Agricultural Produts Quality and Safety,Dongguan 523086,China;Agriculture Research Center of Dongguan,Dongguan 523086,China)
出处
《化学试剂》
CAS
北大核心
2019年第4期392-397,共6页
Chemical Reagents
