摘要
为了解秦皇岛昌黎地区毛皮动物大肠杆菌毒力基因和耐药基因的分布及药物敏感性情况,以60只临床患病毛皮动物为试验菌株的分离宿主,采用常规分离纯化、生化鉴定以及分子生物学的方法对病原菌进行鉴定,动物致病性试验检测其致病性,PCR方法检测毒力基因和耐药基因,K-B法对常用西药进行药物敏感性检测,琼脂平板打孔法对中草药药物敏感性进行检测,试管二倍稀释法检测中草药的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果显示:此次分离到的28株大肠杆菌均使小鼠发病死亡;其中,24株大肠杆菌检测到irp2、fyuA毒力基因,检出率为85.7%;5株大肠杆菌临床分离菌株检出Ler、eaeA毒力基因,检出率为17.9%;耐药基因blaTEM、aadA1、blaCTX-M、blaTEM1、blaSHV-1、strA-strB、blaPSE1和blaOXA1的检出率分别为85.7%,89.3%,75.0%,71.4%,25.0%,24.1%,14.3%,14.3%;西药药敏试验结果显示,28株菌对链霉素、头孢拉定、多西环素、复方新诺明、林可霉素、替米考星、青霉素同时耐药;中草药药敏试验结果显示,诃子、乌梅、五味子对这28株大肠杆菌均具有较好的体外抑菌效果,抑菌圈直径为18～31 mm,MIC和MBC为15.63～500.00 g/L。
In order to understand the distribution of virulence genes and drug resistance genes and drug sensitivity in fur animal E.coli strains from Changli,Qinhuangdao,60 diseased fur-bearing animals were used as the isolation hosts for the test strains,and methods of routine isolation,purification,biochemical identification,and molecular biological analysis were used to identify pathogenic bacteria,animal pathogenicity test was used to detect E.coli pathogenicity,PCR methods were used to detect virulence genes and drug resistance genes,K-B methods were used to detect drug susceptibility of commonly used antibiotic,agar plate drilling method was used to detect the sensitivity of Chinese herbal medicines,and test tube double dilution method was used to detect the minimum inhibitory concentration(MIC) and the minimum bactericidal concentration(MBC) of Chinese herbs.The test results showed that 28 strains of E.coli were isolated and detected,which further resulted in the occurrence of serious illness and death in experimental mice after intraperitoneal injection;the detection rate of virulence genes of Ler and eaeA virulence genes were 14.3% and 14.3%,respectively;the detection rates of blaTEM,aadA1,blaCTX-M,blaTEM1,blaSHV-1,strA-strB,blaPSE1 and blaOXA1 were 85.7%,89.3%,75%,71.4%,25%,24.1%,14.3% and 14.3%,respectively;commonly used antibiotic susceptibility test showed that 28 strains were resistant to streptomycin,cephradine,doxycycline,cotrimoxazole,lincomycin,tilmicosin and penicillin;Chinese herbal medicine susceptibility test showed that Terminalia chebula,Prunus mume,and Schisandra chinensis displayed good antibacterial effect on E.coli strains all in vitro,the inhibition zone diameter were from 18 to 31 mm,and MIC and MBC were from 15.63 to500.00 g/L.
作者
高光平
朱利霞
王洪彬
史秋梅
GAO Guang-ping;ZHU Li-xia;WANG Hong-bin;SHI Qiu-me(Hebei Key Laboratory of Preve? itive Veterinary Medicine丄Heb" Normal University of Science & Technology .Qinhuandao,Hebei 066004 China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2019年第4期671-677,共7页
Chinese Journal of Veterinary Science
基金
河北省自然科学基金资助项目(C2015407033)
河北省教育厅资助项目(SLRC2017037)
河北省高层次人才资助项目(A2016005046)
河北科技师范学院科学研究基金资助项目
关键词
毛皮动物
大肠杆菌
毒力基因
耐药基因
药敏试验
fur-bearing animal
E.coli
virulence genes
drug resistance genes
drug sensitivity test
