摘要
以银杏基因组数据为基础,对银杏bHLH转录因子进行筛选和分析,从银杏基因组中鉴定出72条bHLH转录因子。进一步分析发现,不同bHLH转录因子序列长度和分子量差异较大,而理论等电点及亲水性等比较接近;各家族成员均含有N端碱性氨基酸区和C端的螺旋-环-螺旋区;该家族可分为17个亚家族,相同亚家族成员保守基序的类型十分相似。通过启动子分析发现,多数银杏bHLH基因启动子均含有光响应元件、激素响应元件和逆境胁迫响应元件等。表达分析结果显示,有7条银杏bHLH基因的表达具有组织特异性,有6条基因在各组织中表达水平都比较高,预测其在银杏生物学过程中具有十分重要的作用。
Based on the genome data, the bHLH transcription factors (TFs) of ginkgo were screened and analyzed. At last, 72 bHLH TFs were identified from ginkgo genome. Further analysis revealed that the sequence length and molecular weight of different bHLH TFs were significantly different, while the theoretical isoelectric point and hydrophilic point were relatively close. Each bHLH member contained the N-terminal alkaline amino acid region and the C-terminal helix-loop-helix region. According to the evolution analysis results, all bHLH TFs could be divided into 17 subfamilies, and the same subfamily members were similar in the type of conservative motif. The promoter analysis results indicated that most ginkgo bHLH gene promoters contained light response elements, hormone response elements and stress response elements. The expression analysis results demonstrated that seven ginkgo bHLH genes were tissue-specific and six genes were highly expressed in all tissues, which predicted that they played a very important role in the biological process of ginkgo.
作者
冯磊
石元豹
汪贵斌
曹福亮
FENG Lei;SHI Yuan-bao;WANG Gui-bin;CAO Fu-liang(Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing 210037, China;School of Forestry, Nanjing Forestry University, Nanjing 210037, China)
出处
《江苏农业学报》
CSCD
北大核心
2019年第2期400-411,共12页
Jiangsu Journal of Agricultural Sciences
基金
林业公益性行业科研专项重大项目(201504105)
关键词
银杏
bHLH转录因子
进化分析
表达分析
Ginkgo biloba
bHLH transcription factors
evolution analysis
expression analysis
