摘要
[目的]研究牛初乳冻干粉(LBC)及其产品中表皮生长因子(EGF)、转化生长因子-α(TGF-α)和类胰岛素生长因子-1(IGF-1)含量的变化。[方法]采用冷冻干燥工艺制备0～7 d的LBC和常乳冻干粉(LM),采用酶联免疫分析法测定EGF、TGF-α和IGF-1的含量。[结果]2 d内牛初乳冻干后平均干物质含量为200 g/L。第1天LBC中EGF(7.0 ng/g)、TGF-α(16.8 ng/g)和IGF-1(3.36μg/g)的含量最高,此后快速下降,第7天接近LM水平。第0～4天LBC中EGF、TGF-α和IGF-1的平均含量分别为5.6 ng/g、9.6 ng/g和2.42μg/g,分别为LM的7.0倍、13.7倍和6.1倍。3种牛初乳保健食品的EGF和TGF-α含量显著高于LM和2种市售奶粉。初乳素的EGF和TGF-α含量高于其他2种牛初乳保健食品。初乳素的IGF-1含量为1.11μg/g,极显著高于LM。[结论]研究可为牛初乳产品的进一步开发利用提供理论和试验依据。
[Objective] To study the changes of contents of epidermal growth factor(EGF), transforming growth factor-α(TGF-α) and insulin-like growth factor-1 (IGF-1) in lyophilized bovine colostrums (LBC) during the lactation of 7 days and their contents in bovine colostrums products .[Method] Bovine colostrums from the first day to the 7 th days postpartum and milk were prepared into LBC and LM by the freeze-drying technology, and the contents of EGF, TGF-α and IGF-1 were analyzed by enzyme-linked immunoassay.[Result] The average dry matter content of bovine colostrums within 2 days was 200 g/L by lyophilization. The highest content of EGF(7.0 ng/g), TGF-α(16.8 ng/g) and IGF-1(3.36 μg/g) was found in the LBC of the first day postpartum, then rapidly decreased within 7 days, and the contents on the 7 th day were close to LM levels. The average content of EGF, TGF-α and IGF-1 in LBC within 4 days were 5.6 ng/g, 9.6 ng/g, 2.42 μg/g, which were 7.0, 13.7 and 6.1 times as that in LM. The contents of EGF, TGF-α in three bovine colostrums health-care food (BCHF) were significantly higher than two market milk powder and LM. The contents of EGF, TGF-α in prolactin were significantly higher than other two bovine colostrum health food. The content of IGF-1 in prolactin was 1.11 μg/g, which was significantly higher than that in LM.[Conclusion] The research could provide theoretical and experimental basis for the further development and utilization of bovine colostrum products.
作者
王小军
常萍
牛诗捷
刘亚茹
郁桦
汤正寅
毛宇航
秦寅
闫秋菊
王丽
丁方萍
程光宇
WANG Xiao-jun;CHANG Ping;NIU Shi-jie(College of Life Sciences, Nanjing Normal University, Nanjing,Jiangsu 210023;North China College, Nanjing Normal University, Nanjing,Jiangsu 210023)
出处
《安徽农业科学》
CAS
2019年第9期96-99,共4页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(31500373)
江苏高校品牌专业建设工程(TAPP)项目
关键词
牛初乳冻干粉
牛初乳产品
表皮生长因子
转化生长因子-Α
类胰岛素生长因子-1
酶联免疫分析法
Lyophilized bovine colostrums(LBC)
Bovine colostrums products
Epidermal growth factor(EGF)
Transforming growth factor-α(TGF-α)
Insulin-like growth factor-1(IGF-1)
Enzyme-linked immunoassay
