摘要
针对河南省2017年首例基孔肯雅热病例开展流行病学调查和病原特征分析。采用荧光定量RT-PCR方法诊断病例,采用金黄地鼠肾细胞BHK-21分离基孔肯雅热病毒,测定病毒全基因序列、开展进化分析。分离株CHIKV/Henan001/2017属于ECSA基因型,与2016年中国香港输入株hk02在E1基因区段核苷酸同源性最为接近(99.8%)。流行病学调查和分子进化分析同时证实河南省2017年首例基孔肯雅热病例为输入性病例,病例未在河南境内引起继发流行。
To study the epidemiology and etiology characteristics of first imported Chikungunya fever case in Henan province,China,2017.The patient was confirmed by Chikungunya virus(CHIKV)infected as CHIKV ribonucleotide was continuously detected in his serum specimens.BHK-21 cell line was used for virus isolation,the strain was named CHIKV/Henan001/2017.CHIKV/Henan001/2017 belonged to genotype ECSA.The highest ribonucleotide homology sequence of highly conserved region E1 with CHIKV/Henan001/2017 was hk02 strain(99.8%),who was an imported strain to Hong Kong,China,2016.Epidemiological information and laboratory testing confirmed it was an imported Chikungunya fever case in Henan province,2017.No secondary case has been reported.
作者
李幸乐
李懿
王若琳
张白帆
苏佳
郭大城
许汴利
黄学勇
Li Xingle;Li Yi;Wang Ruolin;Zhang Baifan;Su Jia;Guo Dacheng;Xu Bianli;Huang Xueyong(Institute for Infectious Disease Control and Prevention,Henan Center for Disease Control and Prevention,Zhengzhou 450016,China)
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2019年第4期415-418,共4页
Chinese Journal of Preventive Medicine
基金
国家自然科学基金(81573204)
河南省科技创新人才基金(16410051008).
关键词
切昆贡亚病毒
虫媒病毒
基孔肯雅热
全基因测序
Chikungunya virus
Arboviruses
Chikungunya fever
Complete gene sequencing
