摘要
目的评价血清S100B蛋白和神经元特异性烯醇化酶(NSE)在新生儿感染性脑损伤中的诊断价值。方法选择2017年3月至2018年3月蚌埠医学院第一附属医院新生儿重症监护病房(NICU)收治并确诊为感染性脑损伤的足月新生儿65例作为观察组;并选择同期住院患有感染性疾病而无脑损伤的足月新生儿27例作为对照组。利用电化学发光法检测两组血清S100B蛋白和NSE水平;绘制受试者工作特征(ROC)曲线分析S100B蛋白、NSE及二者联合检测诊断新生儿感染性脑损伤ROC曲线下面积、灵敏度和特异度;并分析观察组S100B蛋白、NSE与炎症指标降钙素原(PCT)、超敏C反应蛋白(hs-CRP)和白细胞(WBC)的相关性。结果观察组血清S100B蛋白和NSE水平均显著高于对照组(P<0.05)。血清S100B检测诊断新生儿感染性脑损伤的最佳临界值、灵敏度和特异度分别为418ng/L、81.5%和92.6%,血清NSE检测的最佳临界值、灵敏度和特异度分别为16.54μg/L、56.9%和96.3%,二者联合检测的灵敏度和特异度分别为87.7%、96.3%。血清S100B、NSE检测ROC曲线下面积分别为0.896和0.769,二者联合检测ROC曲线下面积为0.953。S100B蛋白检测ROC曲线下面积大于NSE检测(Z=2.147,P<0.05),二者联合检测ROC曲线下面积大于S100B(Z=2.43,P<0.05)和NSE检测(Z=4.008,P<0.05)。观察组血清S100B蛋白、NSE水平与PCT、hs-CRP、WBC之间均无相关性(P>0.05)。结论血清S100B蛋白和NSE均可作为新生儿感染性脑损伤标志物,S100B蛋白诊断价值优于NSE,二者联合检测诊断价值优于单个标志物检测。
Objective To evaluate the diagnostic value of serum S100B protein and neurone specific enolase(NSE)in neonatal infectious brain injury.Methods A total of 65 full-term neonates definitely diagnosed infectious brain injury in neonatal intensive care unit(NICU)of the First Affiliated Hospital of Bengbu Medical College from Mar 2017 to Mar 2018 were selected as observation group and 27 full-term neonates diagnosed infectious diseases without brain injury in the same period were selected as control group.The levels of serum S100B protein and NSE were measured with electro-chemiluminescence immunoassay.The ROC curves were drawn and calculating the sensitivity,specificity of detection and the area under the ROC curve.Correlation of serum S100B protein,NSE with procalcitonin(PCT),hypersensitive C reactive protein(hs-CRP)and white blood cell(WBC)were analyzed.Results The levels of serum S100B protein and NSE in observation group were significantly higher than those in control group(P<0.05).The best threshold value,sensitivity,specificity of serum S100B protein detection for diagnosis of neonatal infectious brain injury were 418 ng/L,81.5%,92.6%.The best threshold value,sensitivity,specificity of diagnosis of serum NSE detection were 16.54μg/L,56.9%,96.3%.The area under the ROC curve of serum S100B protein and NSE detection were 0.896 and 0.769,respectively.The area under the ROC curve of combination of serum S100B and NSE detection was 0.953.Furthermore,the area under the ROC curve of serum S100B protein detection was significantly larger than that of NSE detection(Z=2.147,P<0.05),and the area under the ROC curve of combination of serum S100B and NSE detection was significantly larger than that of serum S100B detection(Z=2.43,P<0.05)and that of serum NSE detection(Z=4.008,P<0.05).No correlation was found between the S100B protein,NSE and the PCT,hs-CRP,WBC in observation group(P>0.05).Conclusion S100B Protein and NSE could be as markers of neonatal infectious brain injury,and the diagnostic value of serum S100B protein is better than that of serum NSE,and the diagnostic value of the combination detection is better than that of single marker detection.
作者
刘梅
王凤超
梅传忠
朱安友
LIU Mei;WANG Fengchao;MEI Chuanzhong;ZHU Anyou(Department of Clinical Laboratory,the First Affiliated Hospital of Bengbu Medical College,Bengbu,Anhui 233004,China;Department of Laboratory Medicine,Bengbu Medical College,Bengbu,Anhui 233030,China)
出处
《检验医学与临床》
CAS
2019年第9期1232-1234,1239,共4页
Laboratory Medicine and Clinic
