摘要
【目的】检测浙江省40份水稻栽培品种和6份稻瘟病菌生理小种鉴别品种中6个抗性基因分布情况,同时鉴定品种苗瘟抗性水平,探讨携带抗性基因和抗性水平相关性。【方法】利用功能性分子标记检测品种中Pi1、Pi9/Piz、Pi2/Pizt、Pikh、Pikm、Pita,6个稻瘟病抗性基因的分布情况。用2015-2017年田间采集到的141个稻瘟菌菌株鉴定46个水稻品种苗瘟抗性水平。【结果】6个抗性基因在浙江省栽培品种中分布频率不同,其中Pita基因分布最广,占47.83%;其次是Pikh,占41.30%;抗性基因Pi2/Pizt和Pikm在栽培品种中分布频率相等,均为34.78%;抗性基因Pi9/Piz在栽培品种中分布较少,仅有21.74%;抗性基因Pi1仅在稻瘟病菌生理小种鉴别品种TTP中检测到。抗性水平鉴定结果显示22个品种的抗性频率在60%以上,两个品种的抗性频率达到80%以上。【结论】浙江40份水稻栽培品种和6份稻瘟病菌生理小种鉴别品种携带抗性基因和其抗性水平部分相关。
【Objective】The distribution of 6 resistance genes in 40 cultivated rice varieties in Zhejiang(CRZ)and 6 physiological race identification(PRI)cultivars of Magnaporthe grisea as well as the blast resistance of the rice seedlings were studied.【Method】The distribution of 6 resistance genes,i.e.,Pi1,Pi9/Piz,Pi2/Pizt,Pikh,Pikm and Pita,in the rice varieties was obtained using functional markers.The resistance to M.grisea of all 46 rice varieties was determined based on the 141 strains collected in the field from 2015 to 2017.【Result】Among the resistance genes,Pita had the highest distribution frequency at 47.83% followed by Pikh at 41.30%;Pi2/Pizt and Pikm,same at 34.78%;Pi9/Piz,low at 21.74%;and Pi1 found only in TTP,one of PRI cultivars.In all,there were 22 rice varieties that showed a resistance rate beyond 60% ,and 2 varieties more than 80% .【Conclusion】The resistance genes carried by the 40 CRZ and the 6 PRI cultivars partially correlated with the resistance level exhibited by the cultivars.
作者
何海燕
邱海萍
柴荣耀
毛雪琴
王艳丽
孙国昌
HE Hai-yan;QIU Hai-ping;CHAI Rong-yao;MAO Xue-qin;WANG Yan-li;SUN Guo-chang(Zhejiang Academy of Agricultural Sciences,Zhejiang Key Laboratory of Plant Pest Contro-Provincial and Ministerial Co-construction of National Key Laboratory Cultivation base,Hangzhou,Zhejiang 310021,China;Graduate School of Chinese Academy of Agricultural Sciences,Beijing 100080,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2019年第2期214-222,共9页
Fujian Journal of Agricultural Sciences
基金
国家自然科学基金项目(31470249)
国家重点研发计划项目(2016YFD0300706
2016YFD0200804)
浙江省"三农六方"科技协作项目(CTZB-F160728AWZ-SNY1-6)
浙江省重点研发计划项目(2016C02050-3)
湖南省农业科学院科技创新项目(2017JC08)
关键词
水稻
苗瘟
分子标记
抗病基因
rice
rice leaf blast
molecular marker
resistance gene
