摘要
目的:探讨错配修复蛋白MSH2、MSH6、MLH1及PMS2在结直肠癌(Colorectal cancer,CRC)组织中的表达及其临床病理意义。方法:应用免疫组化PV法检测结直肠癌组织(191例)中MSH2、MSH6、MLH1及PMS2的表达情况,将4种MMR蛋白中的1种及以上表达缺失判定为错配修复基因缺陷(dMMR),全部阳性判定为错配修复基因完整(pMMR),并分析其与临床病理特征的关系。结果:(1)191例中pMMR组159例,MMR蛋白表达率83.2%,dMMR组32例,MMR蛋白缺失率为16.8%。MSH2、MSH6、MLH1及PMS2的表达缺失率分别为2.6%、2.6%、8.9%及14.7%;其中共同缺失表达类型为MSH2-MSH6、MLH1-PMS2及4种共同缺失者分别为4例(2.1%)、14例(7.3%)、2例(1.0%)。(2)CRC癌患者dMMR与pMMR在肿瘤部位、肿瘤直径、分化程度等临床病理特征方面比较,差异均有统计学意义(P<0.05),而在性别、年龄、浸润深度、淋巴结转移、脉管侵犯和神经侵犯等方面比较,差异均无统计学意义(P>0.05)。结论:MMR蛋白与CRC临床病理特征关系密切。MMR蛋白对预测CRC的恶性程度、临床预后及发病机制方面可能有指导意义。
Objective:To investigate the expression of mismatch repair protein( MLH1,MSH2,MSH6 and PMS2)with clinicopathological features in colorectal cancer.Method:The expression of mismatch repair protein MLH1,MSH2,MSH6 and PMS2 were determined by immumohistochemistry in 191 cases of CRC. Deletion of one or more of the four MMR proteins were identified as mismatch repair gene defects(dMMR),and all positives were mismatch repair gene integrity(pMMR),their relationship with clinicopathological features was analyzed.Result:Negative staining of MMR proteins was found in 32 cases analyzed.The frequency of loss expression in MSH2,MSH6,MLH1and PMS2 was 2.6%,2.6%,8.9% and 14.7% respectively.Among them,double proteins absence were found in MSH2/MSH6 in 4 cases(2.1%)and MLH1/PMS2 in 14 cases (7.3%).There 2 cases(1.0%)with 4 protein deletion.Patients with dMMR and pMMR in CRC had difference in tumor location,tumor diameter and degree of differentiation(P<0.05),but had no obvious difference in gender,age,invasion depth,lymph node metastasis,vascular invasion and neurological invasion(P>0.05). Conclusion:There is a close relationship between MMR proteins and the clinicopathological characteristics of colorectal cancer.MMR proteins may be biological indicators for estimating malignant degree,clinical prognosis and pathogenesis mechanism of CRC.
出处
《中国医学创新》
CAS
2019年第13期112-115,共4页
Medical Innovation of China
基金
深圳市南山区卫生科技计划项目(2018068)
