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高效液相色谱法同时测定肿痛安胶囊中3种皂苷类成分含量

Simultaneous Determination of Three Kinds of Saponins in Zhongtong'an Capsules by HPLC
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摘要 目的建立测定肿痛安胶囊中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1含量的高效液相色谱法。方法色谱柱为Agilent Eclipse XDB-C18柱(150 mm×4. 6 mm,5μm),流动相为乙腈-水(梯度洗脱),流速为1. 0 m L/min,柱温为30℃,检测波长203 nm,进样量为10μL。结果三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1质量浓度线性范围分别为15. 25~487. 80μg/m L,11. 57~370. 30μg/m L,11. 44~366. 20μg/m L,r> 0. 999 4 (n=5),精密度、重复性和稳定性试验结果的RSD均小于3%,加样回收率为99. 28%~100. 22%,RSD为0. 98%~1. 49%(n=6)。结论该方法简便、快速、准确、可靠,专属性较强,可用于肿痛安胶囊的质量控制。 Objective To establish an HPLC method for the content determination of notoginsenoside R1,ginsenoside Rg1,ginsenoside Rb1 in Zhongtong’an Capsules. Methods The chromatographic column was Agilent Eclipse XDB-C18 column( 150 mm × 4. 6 mm,5 μm),the mobile phase was acetonitrile-water( gradient elution),the flow rate was 1. 0 m L/min,the column temperature was 30 ℃,the detection wavelength was 203 nm and the sample size was 10 μL. Results The notoginsenoside R1,ginsenoside Rg1,ginsenoside Rb1 showed good linearity in the ranges of 15. 25-487. 80 μg/m L,11. 57-370. 30 μg/m L,11. 44-366. 20 μg/m L,r > 0. 999 4( n = 5). The RSDs of the precision,repeatability and stability tests were less than 3%,the average recovery rates were in the range of 99. 28%-100. 22%,RSDs were in the range of 0. 98%-1. 94%( n = 6). Conclusion The method is simple,rapid,accurate and reliable,and has strong specificity. It can be used for the quality control of Zhongtong’an Capsules.
作者 姜燕 戴丽娜 JIANG Yan;DAI Lina(Lianyungang Food Drug Inspection Center,Lianyungang,Jiangsu,China 222006)
出处 《中国药业》 CAS 2019年第10期23-25,共3页 China Pharmaceuticals
关键词 高效液相色谱法 肿痛安胶囊 三七皂苷R1 人参皂苷RG1 人参皂苷RB1 含量测定 HPLC Zhongtong’an Capsules notoginsenoside R1 ginsenoside Rg1 ginsenoside Rb1 content determination
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