摘要
利用静态-动态吸附和解析的方法,从6种树脂中选出了适合纯化头花蓼总黄酮的树脂,同时探讨了其纯化头花蓼总黄酮的条件。结果表明:D101树脂纯化头花蓼总黄酮的效果最好,其纯化条件为:进样液黄酮浓度0. 8 mg/mL并且pH 5,1. 0 mL/min的进样速度,洗脱剂为3. 5倍柱体积70%的乙醇溶液,洗脱流速为2. 0 mL/min。纯化后黄酮的纯度可达81. 93%。
In this paper,6 types of macroporous resins were tested by static and dynamic adsorption and desorption experiments for purifying flavonoids from Polygonum capitatum. And the purifying conditions by the best resin were also investigated. The results showed that D101 resin was the best resin.The adsorption conditions were shown as follows: 1. 0 mL/min adsorption velocity,concentration of sample 0. 8 mg/m L and pH 5. The desorption conditions were shown as follows: 70% ethanol,3. 5 times volume of resin as eluent and the desorption speed of 2. 0 mL/min. After purified with D101 macroporous resin,the purity of the purified Polygonum capitatum flavonoids was up to 81. 93%.
作者
廖莉玲
师仲
顾曼琦
郑奎玲
周清娣
LIAO Liling;SHI Zhong;GU Manqi;ZHENG Kuiling;ZHOU Qingdi(School of Chemistry and Materials Science, Guizhou Normal University, Guiyang, Guizhou 550001, China;School of Chemistry, University of Sydney, Sydney 2006, Australia)
出处
《贵州师范大学学报（自然科学版）》
CAS
2019年第3期13-17,共5页
Journal of Guizhou Normal University：Natural Sciences
基金
教育部春晖计划(编号:Z2016012)
贵州省科技厅与贵州师范大学联合基金项目(黔科合LH字[2014]7064号)
关键词
头花蓼
总黄酮
纯化
大孔树脂
Polygonum capitatum
flavonoids
purification
macroporous resin
