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36种中药对罗非鱼源无乳链球菌体外抑菌及生物被膜消除效果 被引量:14

Antimicrobial and biofilm-eliminated effects of 36 Chinese medicine compounds on Streptococcus agalactiate isolated from tilapia in vitro
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摘要 为了筛选出对无乳链球菌( Streptococcus agalactiate)有抗菌效果的安全新药物,实验采用琼脂扩散法和微量稀释法测定了36种中药单体对3株罗非鱼致病性无乳链球菌的抑菌活性,测定了血根碱对无乳链球菌生长曲线和生物被膜成膜的影响。结果显示:受试菌株对血根碱、白屈菜红碱、厚朴酚、和厚朴酚和大黄酸极度敏感,抑菌圈均在20 mm以上,5种药物对无乳链球菌的最小抑菌浓度( MIC)为8~32 μg/mL,表现出很强的抑制作用,其中血根碱对3株无乳链球菌的最小杀菌浓度( MBC)均为16 μg/mL;生长曲线及生物被膜试验发现血根碱对无乳链球菌生长和生物被膜生成具有抑制作用。在不同血根碱浓度下,与其他浓度组相比当血根碱浓度达到16 μg/mL及以上时,受试菌株生物被膜的生成率降低到2.76%以下,相同条件下,绿原酸作用下生物被膜生成率均在90%以上。 The antimicrobial activity of 36 natural compounds against 3 pathogenic Streptococcus agalactiate strains isolated from diseased tilapia by disc agar diffusion and broth micro-dilution method were investigaeted, to find out new safe drugs which have antimicrobial effect against. S. agalactiate . The effects of S. agalactiate growth curve and biofilm formation with sanguinarine were also determined. The result showed that all tested stains were extremely sensitive to sanguinarine, chelerythrine, magnolol, honokiol and rhein.The inhibitory zone of 5 compounds exceeded 20 mm. The minimal inhibitory concentration ( MIC)of 5 drugs were ranged from 8 to 32 μg/mL. The minimum bactericidal concentration ( MBC) of sanguinarine against 3 S. agalactiae strains were 16, 32 and 16 μg/mL, respectively. Further growth curve and biofilm test implied different sanguinarine concentrations had different antimicrobial effects on the growth and the biofilm formation of S. agalactiate . Compared with other concentrations, the biofilm formation of S. agalactiate was less than 2.76% once sanguinarine concentration reached to 16 μg/mL, and the biofilm formation rate of S. agalactiate was over 90% with chlorogenic acid at the same concentration gradient.
作者 丁浩 董靖 宋怿 苏志俊 余琳雪 艾晓辉 DING Hao;DONG Jing;SONG Yi;SU Zhi-jun;YU Lin-xue;AI Xiao-hui(Shanghai Ocean University ,Shanghai 201306, China;Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences,Wuhan 430223, China;Chinese Academy of Fishery Sciences,Beijing 100125, China;Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing 100125, China;Hubei Province Engineering and Technology Research Center of Aquatic Product Quality and Safety, Wuhan 430223, China)
出处 《淡水渔业》 CSCD 北大核心 2019年第3期71-77,共7页 Freshwater Fisheries
基金 现代农业产业技术体系专项资金资助(CARSD-49)
关键词 无乳链球菌(Streptococcus agalactiate) 生物被膜 生长曲线 血根碱 抑菌作用 Streptococcus agalactiate biofilm growth curve sanguinarine antimicrobial effect
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