摘要
目的探讨天然抗ABCC3 IgG抗体对口腔鳞癌细胞CAL27和SCC15的影响。方法采用ELISA方法筛查富含天然抗ABCC3 IgG抗体的阳性血浆为实验组,低含天然抗ABCC3 IgG抗体的阴性血浆为对照组。采用qRT-PCR检测ABCC3基因的mRNA表达水平。CCK-8实验和流式细胞术检测天然抗ABCC3 IgG抗体对CAL27和SCC15细胞的增殖、凋亡和细胞周期的影响。结果在CAL27和SCC15细胞中均有ABCC3基因的表达。与阴性对照血浆相比较,富含ABCC3 IgG抗体的阳性血浆可以明显抑制CAL27细胞的增殖(P<0.01),促进CAL27细胞的凋亡(P<0.01),并对CAL27细胞周期中的G2/M期产生阻滞作用(P<0.01),但对SCC15细胞无明显影响。结论天然抗ABCC3 IgG抗体对口腔鳞癌细胞的增殖有抑制作用,可能成为治疗口腔鳞癌的一种有前景的新药物。
Objective To investigate the effect of natural anti-ABCC3 antibodies on oral squamous cell carcinoma (OSCC) cell lines, CAL27 and SCC15. Methods An in-house enzyme-linked immune-sorbent assay (ELISA) was performed to detect the anti-ABCC3 IgG antibodies in human plasma and then CAL27 and SCC15 cells were treated with plasmas. Plasma with low anti-ABCC3 IgG antibodies was used as control and ABCC3 expression was identified by quantitative RT-PCR. CCK-8 assay and flow cytometry was used to evaluate the effect of anti-ABCC3 IgG antibodies on proliferation, apoptosis and cell cycle of cells. Results The plasma rich in natural anti-ABCC3 IgG antibodies significantly inhibited the proliferation of CAL27 cells ( P <0.01), but had no remarkable suppressive effect on SCC15 cells though the expression of ABCC3 gene was found in both CAL27 and SCC15 cells. It could also reduce the apoptosis of CAL27 cells ( P <0.01) and arrest the G2/M phase of CAL27 cell cycle ( P <0.01). Conclusion The natural anti-ABCC3 IgG antibodies may be a potential therapy for the treatment of OSCC.
作者
刘秀
黄志成
董蕊
何子毅
孟庆勇
胡颖
LIU Xiu;HUANG Zhi-cheng;DONG Rui;HE Zi-yi;MENG Qing-yong;HU Ying(Beijing Institute of Dental Research, Capital Medical University School of Stomatology, Beijing 100050, China)
出处
《北京口腔医学》
CAS
2019年第2期67-71,共5页
Beijing Journal of Stomatology
基金
国家自然科学基金(81570958)
北京市自然科学基金(7162075)
关键词
天然抗体
ABCC3
口腔鳞癌
Natural antibody
ABCC3
Oral squamous cell carcinoma
