摘要
目的:建立灵芝及其相关产品中麦角甾醇物质含量的测定方法。方法:采用高效液相色谱(HPLC)方法定量分析灵芝及其相关产品中麦角甾醇物质的含量。色谱柱为Agilent prep-C18 (250 mm×4.6 mm,5μm),流动相:98%色谱甲醇,流速:1.0 mL/min,检测波长:282 nm,柱温:28℃。结果:麦角甾醇在20～200μg/mL呈良好的线性关系,线性回归方程为:y=13.32x-24.32(r=0.999 7),平均加样回收率为98.50%～99.93%,RSD为0.5%。结论:本法操作简单、结果准确、重现性好,为灵芝及其相关产品的质量控制提供参考方法。
Objective To establish a method for determination of ergosterol in Ganoderma lucidum and its related products. Methods the content of ergosterol in Ganoderma lucidum and its related products was used to quantitatively analyze by High performance liquid chromatography(HPLC). The separation was performed on an Agilent prep-C18 column,with 98% Chromatographic methanol as the mobile phase at a flow rate of 1.0 mL/min. The Detection wavelength was 282 nm;the column temperature was 28℃. Results Ergosterol showed a good linear relationship within the range of 20~200 μg/mL. The linear regression equation is :y=13.32x-24.32(r=0.9997).The average sample recovery rate is 98.50%-99.93%, and RSD is 0.5%. Conclusions The method is simple、accurate and credible,that can provide a reference method for the quality control of Ganoderma Lucidum and its related products.
作者
罗翠婷
颜舒柳
张一帆
LUO Cuiting;YAN Shuliu;ZHANG Yifan(Zhanjiang Chinese Medicine School, Zhanjiang 524094,China;Guangdong Institute of Microbiology, Guangzhou 510070,China)
出处
《中国民族民间医药》
2019年第7期39-41,共3页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
广东省农业标准化专项资金资助的项目(粤财农[2012]528号)
关键词
灵芝
麦角甾醇
HPLC
Ganoderma Lucidum
Ergosterol
HPLC
