摘要
研究急性淋巴细胞白血病(ALL)患儿血清中miR-17、miR-29c表达水平及其与患儿生存率的关系。方法:选择 2010年9月~2013年2月在笔者医院初诊为ALL并住院治疗的43例患儿作为病例组,同时期体检健康儿童40例作为对照组。采用RT-PCR检测两组儿童血清中miR-17、miR-29c相对表达量,分析病例组血清miR-17、miR-29c表达水平与患儿初诊白细胞(WBC)计数、免疫分型等临床指标及患儿5年无事件生存率、总生存率的关系。结果:病例组儿童血清中miR-17相对表达量显著高于对照组( P <0.05),miR-29c相对表达量显著低于对照组( P <0.05);病例组血清中miR-17表达水平与患儿初诊WBC计数、免疫分型有关( P <0.05),miR-29c表达水平与患儿初诊WBC计数、免疫分型及泼尼松诱导敏感度有关( P <0.05);病例组患儿5年无事件生存率51.16%,总生存率72.09%;生存组患儿血清miR-17相对表达量显著低于死亡组患儿( P <0.05),miR-29c相对表达量显著高于死亡组患儿( P <0.05);miR-17高表达组患儿5年无事件生存率、总生存率均显著低于miR-17低表达组患儿;miR-29c高表达组患儿5年无事件生存率、总生存率均显著高于miR-29c低表达组患儿。结论:ALL患儿血清中miR-17表达上调,miR-29c表达下调,与患儿预后不良有关。
To study the expression levels of miR-17 and miR-29c in serum of children with acute lymphoblastic leukemia(ALL)and their relationships with the survival rate of children. Methods Totally 43 cases of children who diagnosed as ALL and hospitalized in our hospital from September 2010 to February 2013 were selected as case group, and 40 healthy children were used as control group at the same time. RT-PCR was used to detect the relative expressions of miR-17 and miR-29c of children in two groups. The relationships between the levels of miR-17 and miR-29c expression in the case group with the clinical indexes of leukocyte (WBC) count and immunophenotype when first diagnosed, and the 5-year event free survival rate and the total survival rate of the children were analyzed. Results The relative expression of miR-17 in serum of children in case group was significantly higher than that in control group ( P <0.05). The relative expression of miR-29c was significantly lower than that in the control group ( P <0.05). The expression level of miR-17 in serum of case group was related to WBC count and immunophenotype ( P <0.05). The miR-29c expression level was correlated with the WBC count, immunophenotype and prednisone induction sensitivity ( P <0.05). The 5-year event free survival rate of case group was 51.16%, and overall survival rate was 72.09%. The relative expression level of serum miR-17 in the survival group was significantly lower than that in the death group ( P <0.05). The relative expression of miR-29c was significantly higher than that in the death group ( P <0.05). The 5-year event free survival rate and overall survival rate of miR-17 high expression group were significantly lower than those of miR-17 low expression group. The 5-year event free survival rate and overall survival rate of miR-29c high expression group were significantly higher than those of miR-29c low expression group. Conclusion Up-regulation of miR-17 expression and down-regulation of miR-29c expression in serum of children with ALL are associated with poor prognosis.
作者
赵丽颖
李慧霞
张红梅
Zhao Liying;Li Huixia;Zhang Hongmei(Department of Hematology,Children′s Hospital Affiliated of Zhengzhou University,Henan Children′s Hospital,Henan 450053,China)
出处
《医学研究杂志》
2019年第4期111-116,共6页
Journal of Medical Research
