摘要
以金针菇麦角硫因为研究对象,分析提取方法、液料比、乙醇浓度、提取温度等单因素对其提取量的影响,在此基础上采用响应面法优化金针菇麦角硫因的提取工艺条件。结果表明:不同的提取方法可显著影响(p<0.05)金针菇麦角硫因的提取量,其中,溶剂法提取的麦角硫因提取量较高,且具有节约成本和可操作性好的优势;单因素和响应面法得到的溶剂法提取麦角硫因的最佳工艺条件为:提取时间15 min,乙醇浓度62%,液料比58∶1 (mL/g),温度53℃;以该条件进行的验证实验所得麦角硫因的提取量为(3.53±0.09) mg/g干重,与预测值(3.48 mg/g干重)无显著差异(p>0.05),表明优化得到的工艺具有有效性和可靠性。
Effects of single factors,including extraction method,liquid-solid ratio,ethanol concentration and extraction temperature,on extraction rate of Flammulina velutipes ergothioneine were studied in this manuscript.Based on single factor experiments,the optimum process conditions for the ergothioneine extraction were determined using response surface methodology.The results showed that the different extraction methods could significantly(p<0.05)affect the extraction yield of EGT from Flammulina velutipes,of which the solvent extraction had the higher extraction yield and was considered as convenient and low energy consumption process for ergothioneine;The optimum extraction conditions obtained through response surface methodology were as follows:extraction time of 15 min,ethanol concentration of 62%,liquid-solid ratio of 58∶1(g/mL),extraction temperature of 53℃;Verification experiment was carried out under the optimum extraction,and the highest ergothioneine yield was(3.53±0.09)mg/g dry weight.No significant difference was found between observed and estimated values(3.48 mg/g dry weight),suggesting that the optimized process was reliable and valid for Flammulina velutipes ergothioneine extraction.
作者
胡晶晶
张怡馨
王艳
莫宇丽
王杰
HU Jing-jing;ZHANG Yi-xin;WANG Yan;MO Yu-li;WANG Jie(College of Food Science,South China Agricultural University,Guangzhou 510642,China)
出处
《食品工业科技》
CAS
北大核心
2019年第9期171-177,共7页
Science and Technology of Food Industry
基金
国家自然科学基金项目(31772020)
广州市科技计划项目(2014J4100189)
关键词
金针菇
麦角硫因
提取工艺
响应面法
Flammulina velutipes
ergothioneine
extraction process
response surface methodology
