摘要
通过对3个地区、6种植物采集的7种植物枯萎病病原菌的分离纯化,并运用真菌形态观察法和r DNA-ITS分子生物学技术进行了鉴定。形态鉴定结果表明,不同枯萎病病原菌在PDA培养基上均会产生色素,菌丝大多致密,分生孢子大多呈弯月形,菌丝有隔。通过进一步的分子学鉴定,鉴定结果分别为辣椒枯萎菌、兰花枯萎菌、番茄枯萎菌1为镰刀菌;番茄枯萎菌2、甜瓜枯萎菌、西瓜枯萎菌为尖孢镰刀菌;茄子枯萎菌为拟轮枝镰刀菌。枯萎病主要的侵染真菌是尖孢镰刀菌和镰刀菌,少数为拟轮枝镰刀菌。研究结果可为今后对枯萎真菌病害的鉴定提供理论依据。
Plant fusarium wilt is one of the most important disease threats in the world, which mainly endangers the production of tomato, watermelon and melon. Seven pathogenic bacteria of plant wilt were isolated and purified from three areas and six plants, and identified by fungal morphological observation and rDNA-ITS molecular biology techniques. The results of morphological identification showed that different fusarium wilt fungi could produce pigments on PDA medium. The mycelia were mostly dense, conidia were mostly meniscus-shaped, and the mycelia were septated. Through further molecular identification, the identification results showed that Capsicum annuum L. fusarium, Cymbidium usarium, Lycopersicon esculentum M. fusarium 1 were Fusarium redolens. Lycopersicon esculentum M. fusarium 2, Cucumis melo fusarium, Citrullus lanatus fusarium were Fusariumo xysporum. Solanum melongena L. fusarium was Fusarium verticillium. Fusarium oxysporum and Fusarium redolens are the main infective fungi of fusarium wilt, and a few are Fusarium verticillium. The results of this study provide a theoretical basis for the identification of fusarium wilt fungal diseases in the future.
作者
郑瑞瑞
安丽
何晓婷
郝晓娟
曹挥
ZHENG Ruirui;AN Li;HE Xiaoting;HAO Xiaojuan;CAO Hui(College of Agronomy,Shanxi Agricultural University,Taigu 030801,China)
出处
《山西农业科学》
2019年第5期904-907,共4页
Journal of Shanxi Agricultural Sciences
基金
山西功能农业共性关键技术研究与示范(201703D211001-01-04)
山西省重点研发项目(201703D211014)
关键词
植物枯萎病原菌
镰刀菌属
形态学观察
分子鉴定
plant fusarium wilt pathogen
Fusarium redolens
morphological observation
molecular identification
