摘要
以湖南长沙和湖北襄阳仓储稻谷为对象,对稻谷储藏期间主要优势霉菌进行分离纯化,记录其在高盐察氏培养基上的菌落形态。通过蔡司显微镜分别拍摄了主要优势霉菌的显微结构特征,并通过主要优势霉菌的DNA提取和测序,在基因水平上对霉菌种类进行了鉴定。经鉴定,主要优势霉菌分别为:黄曲霉、黑曲霉、白曲霉、灰绿曲霉和产黄青霉。
The rice stored in Changsha (in Hunan province) and Xiangyang (in Hubei Province) was chosen as the object to separate the dominant mold from the stored rice and purify them, and record the colony morphology in Salt Czapek Dox Agar. The microstructure characteristics of the main dominant mold were photographed by Zeiss microscope respectively. The main dominant mold were identified at the genetic level by extracting and sequencing the DNA, which were aspergillus flavus, aspergillus niger, aspergillus albicans, aspergillus glaucus and penicillium chrysogenum.
作者
葛志文
方宝庆
邱伟芬
周建新
GE Zhi-wen;FANG Bao-qing;QIU Wei-fen;ZHOU Jian-xin(College of Food Science and Engineering / Modern Grain Logistic and Security Cooperative Innovation Center of Jiangsu Province/Key Laboratory of Grain and Oil Quality Control and Deep Processing Technology of Jiangsu Province,Nanjing University of Finance and Economics,Jiangsu Nanjing 210023)
出处
《粮油食品科技》
2019年第3期72-77,共6页
Science and Technology of Cereals,Oils and Foods
基金
"十三五"国家重点研发计划课题(2016YFD0401003)
关键词
储藏稻谷
优势霉菌
菌落形态
显微结构
菌种鉴定
paddy storage
dominant mold
colony morphology
microstructure
strain identification
