摘要
以N-乙基苯并吲哚与对苯二甲醛缩合产物为底物,其缩合后产物(EFDB)与2,3-二氨基丁烯二腈缩合得新型的半花菁类衍生物(EADB),采用~1H NMR及质谱进行结构表征﹒铁离子诱导EADB荧光信号明显增强,且体系荧光信号的变化与铁离子浓度在2～100 mol/L范围内呈良好的线性关系,检出限为0.54 mol/L﹒采用铁离子介导的"关-开"策略,构建了黑茶抗氧化活性的测定方法,并成功应用于实际样品.
A novel hemicyanine derivatives of EADB was obtained using N-ethyl benzoindoles and p-phthalaldehyde as raw materials,following their condensation product then reacted with 2,3- diaminomaleonitrile.The products were characterized by 1H NMR and HRMS.After addition of Fe^3+,the fluorescent signal were significantly enhanced,and the change in fluorescent intensity was linear with Fe3+ concentration in the range from 2 to 100 mol/L,with a detection limit of 0.54 mol/L.Then,a florescent method was constructed for assaying antioxidant capacity of dark tea by Fe3+-mediated turn-on strategy.
作者
许超
汪楠
夏婷
罗苗
曹一鸣
龙立平
齐风佩
刘长辉
XU Chao;WANG Nan;XIA Ting;LUO Miao;CAO Yiming;LONG Liping;QI Fengpei;LIU Changhui(Hunan Provincial Key Lab of Dark Tea and Jin-hua,College of Materials and Chemical Engineering,Hunan City University,Yiyang,Hunan 413000,China;State Key Laboratory of Chemo/Biosensing and Chemometrics,Hunan University,Changsha,Hunan 410082,China)
出处
《湖南城市学院学报（自然科学版）》
CAS
2019年第3期69-73,共5页
Journal of Hunan City University:Natural Science
基金
国家自然科学基金项目(21675042)
国家大学生创新创业训练计划项目([2017]7)
湖南省自然科学基金项目(2018JJ2017)
湖南省教育厅优秀青年项目(15B043)
湖南省大学生研究性学习和创新性实验计划项目([2017]116)
黑茶金花湖南省重点实验室开放基金(HC6120)
关键词
黑茶
抗氧化活性
铁离子
荧光分析
dark tea
antioxidant capavity
ferric ion
fluorescence analysis
