摘要
种子在长期的贮存过程中会发生不可逆的衰老现象,从而导致品质和产量下降。合理的修复处理可以提高老化种子的活力和幼苗质量。本研究采用四因素[超声温度、超声时间、抗坏血酸(ascrobic acid, AsA)浓度、抗坏血酸浸种时间]三水平L_9(3~4)正交试验,对人工老化的梭梭(Haloxylon ammodendron)种子进行超声波和AsA混合处理,研究超声波和AsA对种子萌发和幼苗生理特性的影响,分析其是否可以修复老化造成的损伤,并选出最优的处理条件。结果表明,适当剂量的超声波和AsA能够对老化种子产生修复作用,处理后种子发芽率、发芽势、发芽指数和幼苗长度均得到显著提高(P <0.05),种子浸出液电导率、丙二醛(MDA)含量和脯氨酸含量下降,可溶性蛋白含量增加,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)等保护酶活性增强,其中超声温度为影响种子活力的最重要因素。研究分析得出,老化梭梭种子的最优修复处理条件为:超声温度25℃,超声时间20 min,AsA浓度300 mg·L^(–1),AsA浸种时间24 h。
Seeds may undergo irreversible aging during long-term storage,causing a decline in the seed quality and yield.Reasonable repair treatments can improve the vigor and quality of aged seeds.In this study,an orthogonal matrix test method was used to study the influence of ultrasound temperature,ultrasound time,ascorbic acid(AsA)concentration,and AsA soaking time,on seed germination and the physiological characteristics of artificially aged Haloxylon ammodendron seeds,to evaluate if there were repair effects on the seeds and to identify optimal treatment conditions.The results indicated that an ultrasound temperature treatment and AsA treatment had positive repair effects on the aged seeds.The treatments significantly promoted(P<0.05)the percentage of seed germinated,the germination potential,germination index,and seedling length.Further,the soaking liquid conductivity was decreased,as were the malondialdehyde(MDA)content and the proline content;however,the soluble protein content,superoxide dismutase(SOD),peroxidase(POD),and the catalase(CAT)activity increased.Ultrasonic temperature is one of the most important factors that can influence seed germination and seed vigor,it was concluded that the optimal treatment conditions for aged Haloxylon ammodendron seeds were an ultrasound temperature of 25℃,an ultrasound exposure time of 20 min,an AsA concentration of 300 mg·L^–1,and an AsA soaking time of 24 h.
作者
赵恬
柳旭
周文楠
潘胜难
张钰靖
钟元
蒋文博
王佺珍
ZHAO Tian;LIU Xu;ZHOU Wennan;PAN Shengnan;ZHANG Yujing;ZHONG Yuan;JIANG Wenbo;WANG Quanzhen(College of Animals Science and Technology,Northwest A&F University,Yangling 712100,Shaanxi,China)
出处
《草业科学》
CAS
CSCD
2019年第5期1333-1343,共11页
Pratacultural Science
基金
国家重点研发计划(2017YFE0111000)
国家自然科学基金(31472138)
关键词
种子老化
梭梭
超声波
抗坏血酸
正交试验
seed aging
Haloxylon ammodendron
ultrasound
ascorbic acid
orthogonal matrix test
