摘要
目的探索热休克蛋白27(Hsp27)、间隙连接蛋白43(CX43)、RhoA、Rho相关激酶(ROCK)Ⅰ、ROCKⅡmRNA和蛋白表达水平在宫缩乏力性产后出血诊断中的价值。方法将2015年9月至2018年4月于该院分娩后发生宫缩乏力性产后出血的产妇纳入观察组(n=89),未发生产后出血的产妇纳入对照组(n=90)。比较两组产妇临床资料,子宫平滑肌收缩频率、幅度和活动力,子宫平滑肌Hsp27、CX43、RhoA、ROCKⅠ、ROCKⅡmRNA与蛋白的表达量,并分析其与子宫平滑肌收缩活动力的相关性。结果两组产妇年龄、体质量指数、产次、新生儿体质量、孕周、凝血4项和国际标准化比值水平比较,差异无统计学意义(P>0.05)。观察组子宫平滑肌收缩频率、幅度和活动力小于对照组,差异有统计学意义(P<0.05)。观察组子宫平滑肌Hsp27、CX43、RhoA、ROCKⅠ、ROCKⅡmRNA和蛋白表达量小于对照组,差异有统计学意义(P<0.05)。Hsp27、CX43、RhoA、ROCKⅠ、ROCKⅡmRNA及蛋白表达量均与子宫平滑肌收缩活动力呈正相关(P<0.05)。结论 Hsp27、CX43、RhoA、ROCKⅠ、ROCKⅡmRNA及蛋白表达量的检测与子宫平滑肌的收缩能力相关,对诊断宫缩乏力性产后出血具有重要价值。
Objective To explore the value of mRNA and protein of heat shock protein 27 (Hsp27),connexin 43 (CX43),RhoA and Rho associated kinase (ROCK)Ⅰ,ROCKⅡ in the diagnosis of postpartum hemorrhage due to uterine inertia. Methods From September 2015 to April 2018,the patients with postpartum hemorrhage due to uterine weakness after deliver in Maternal and Child Health Hospital of Changning District were recruited into observation group ( n =89) and patients without postpartum hemorrhage due to uterine weakness after deliver were recruited into control group ( n =90).The clinical data,frequency,amplitude and activity of uterine smooth muscle contraction,expression of Hsp27,CX43,RhoA,ROCKⅠ,ROCKⅡ mRNA and protein in uterine smooth muscle were compared in them and the correlation between the expression of Hsp27,CX43,RhoA,ROCKⅠ,ROCKⅡ mRNA,protein and the contractile activity of uterine smooth muscle was examined. Results There were no significant differences on age,Body Mass Index,birth time,neonatal weight,gestational age,four items of coagulation and international standardized ratio in the two groups ( P >0.05).The observation group′s contractile frequency,amplitude and motility of uterine smooth muscle were smaller than those in the control group ( P <0.05).The observation group′s Hsp27,CX43,RhoA,ROCKⅠ,ROCKⅡ mRNA and protein in uterine smooth muscle were lower than those in the control group ( P <0.05).The expression of Hsp27,CX43,RhoA,ROCKⅠ,ROCKⅡ mRNA and protein correlated positively with the contractile activity of uterine smooth muscle ( P <0.05). Conclusion The detection of Hsp27,CX43,RhoA,ROCKⅠ,ROCKⅡ mRNA and protein expression related to the contractile activity of uterine smooth muscle,which is important for the diagnosis of postpartum hemorrhage due to uterine atony.
作者
胡谢应
何丽梅
俞菁
顾彦洁
龚波
HU Xieying;HE Limei;YU Jing;GU Yanjie;GONG Bo(Department of Clinical Laboratory,Maternal and Child Health Hospital of Changning District,Shanghai 200051,China)
出处
《检验医学与临床》
CAS
2019年第10期1358-1361,共4页
Laboratory Medicine and Clinic
基金
上海市长宁区卫生和计划生育委员会资助项目(20164Y012)
