摘要
目的探讨黑骨藤乙醇提取物对大鼠坐骨神经钳夹损伤的作用及可能的作用机制。方法将成年雄性SD大鼠120只分为4组,分别为假手术组(A组)、模型组(B组)、黑骨藤10mg/kg组(C组)、黑骨藤20mg/kg组(D组),每组30只。B、C、D组大鼠均行坐骨神经损伤(SNI)手术,A组仅暴露游离坐骨神经,术后每日分别给予0.90%氯化钠(A、B组)或相应剂量的黑骨藤乙醇提取物(C、D组)灌胃。各组分为3个时间点(7、14、28d)测定坐骨神经功能指数(SFI);取坐骨神经,行HE染色观察大鼠坐骨神经的组织形态学变化;取股动脉血,ELISA法检测各组大鼠血清中白细胞介素(IL)-1、IL-6和肿瘤坏死因子-α(TNF-α)的表达水平。结果与A组比较,B组大鼠SFI明显降低(P<0.01);与B组比较,C组大鼠第28天的SFI明显增高(P<0.01),D组大鼠第14、28天的SFI均明显升高(P<0.01)。HE染色显示,C、D组大鼠第7天轴索肿胀比B组减轻,第14天时轴索肿胀减轻明显,且髓鞘变性改善;D组大鼠第28天时神经纤维组织结构基本恢复正常。与A组比较,B组大鼠IL-1、IL-6、TNF-α水平明显升高(P<0.01)。与B组比较,C组大鼠在第28d时TNF-α水平明显降低(P<0.05);D组大鼠在第7、14、28天3个时间点IL-1、IL-6、TNF-α水平均明显降低(P<0.05)。结论黑骨藤乙醇提取物可改善大鼠SNI,其作用机制可能与抑制免疫炎症因子有关。
Objective To study the protective effects of the alcohol extract of Periploca forrestii Schltr(PFS)and the possible mechanisms in rats with sciatic nerve crush lesion.Methods 120 male adult SD rats were divided into 4 groups randomly:the sham group(group A),the model group(group B),the 10 mg/kg PFS group(group C),the 20 mg/kg PFS(group D).Each group was divided into 3 time points(7,14 and 28 days),and 0.90%sodium chloride or the corresponding dose of ethanol extract from PFS were given to gastricgavage every day.The sciatic nerve function index(SFI)was measured and HE staining was used to observe the pathological changes of the sciatic nerve.The changes of interleukin(IL)-1,IL-6,and tumor necrosis factor-alpha(TNF-α)were assessed by ELISA.Results SFI was significantly lower in group B than in group A(P<0.01).Compared with the B group,SFI was significantly higher at the 28 th day in group D(P<0.01)and increased at the 14 th and 28 th day in group C(P<0.01).HE staining indicated that the axonal swelling reduced in group C and D,compared with the model group at the 7 th day.The myelin degeneration was improved at the 28 th day and the structure of nerve fibers recovery at the 28 th day.As compared with group A,the levels of IL-1,IL-6 and TNF-αwere significantly higher in group B(P<0.01).As compared with group B,the levels of TNF-αsignificantly decreased in group D at the 28 th day(P<0.05),IL-1,IL-6 and TNF-αwere all significantly lower in group C at the 7 th,14 th,and 28 th days(P<0.05).Conclusion PFS improved the sciatic nerve injury in rats,which might be correlative to inhibit the inflammatory cytokines.
作者
王恒
吉杨丹
王燕林
党荣敏
余跃生
沈祥春
WANG Heng;JI Yangdan;WANG Yanlin;DANG Rongmin;YU Yuesheng;SHEN Xiangchun(Qiannan Medical College For Nationalities,Duyun,Guizhou 558003,China;Key Laboratory of Optimal Utilization of Natural Medicine Resources,Guizhou Medical University,Guiyang,Guizhou 550025,China)
出处
《重庆医学》
CAS
2019年第9期1489-1492,1499,共5页
Chongqing medicine
基金
贵州省中医药管理局中医药
民族医药科学技术研究项目(QZYY-2018-99)
黔南科技计划项目(黔南科合社字[2017]58号
黔南科合社字[2017]74号)
黔南民族医学高等专科学校校基金项目(QNYZ201713号)
关键词
黑骨藤
坐骨神经损伤
炎性细胞因子
periploca forrestii schltr
sciatic nerve injury
inflammatory cytokines
