摘要
为了探讨大黄素增强体外扩增NK细胞抗肺癌A549细胞杀伤效力的相关机制,体外培养A549肺癌细胞系和体外扩增NK细胞,将大黄素分对照组、低、中、高浓度组(0、1、12. 5、25μg/m L),通过MTT检测不同浓度大黄素在不同作用时间对A549细胞生长的抑制作用;通过钙黄绿素释放实验检测经不同浓度大黄素处理的NK细胞对A549细胞的杀伤效力;通过流式细胞仪检测经不同浓度大黄素处理的NK细胞特异性配体MIC A/B、HLA-ABC的表达。结果显示,大黄素能有效抑制A549细胞生长,增强NK细胞对A549细胞的杀伤效力,上调NK细胞活化性受体相关配体MIC A/B的表达,下调NK细胞抑制性受体相关配体HLA-ABC表达。因此,大黄素可以通过调节MIC A/B、HLA-ABC分子表达,增强体外扩增NK细胞对A549细胞的杀伤效力,为中药单体调节免疫细胞抗肿瘤在临床应用方面提供实验室依据。
The mechanism of emodin of killing lung cancer A549 cells through in vitro expansion NK cells was studied.A549 lung cancer cell lines were cultured in vitro and NK cells were amplified.The inhibiting effect of emodin with concentration of 0,1,12.5 and 25 μg/mL was tested by MTT.The capacity of emodin-trated NK cells of killing A549 cells was tested by calcein-AM release assay.The expressions of MIC A/B and HLA-ABC in NK cells treated with emodin at different concentrations were detected by flow cytometry.The results showed that emodin could effectively inhibit the growth of A549 cells and enhance the killing effect of NK cells on A549 cells.It could up- regulate the expression of MIC A/B, an activated receptor-associated ligand of NK cells, and down-regulate the expression of HLA-ABC, an inhibitory receptor-associated ligand of NK cells.It is concluded that emodin can enhance the killing effect of NK cells on A549 cells in vitro by regulating the expression of MIC A/B and HLA-ABC molecules.
作者
王晓燕
刘畅
朴星虎
宋伍
王微
白金萍
王冰梅
WANG Xiao-yan;LIU Chang;PIAO Xing-hu;SONG Wu;WANG Wei;BAI Jin-ping;WANG Bing-mei(Clinical Medical College, Changchun University of Chinese Medicine, Changchun 130117,China;InnovationPractice Center, Changchun University of Chinese Medicine,Changchun 130117,China)
出处
《辽东学院学报（自然科学版）》
CAS
2019年第2期102-106,共5页
Journal of Eastern Liaoning University:Natural Science Edition
基金
吉林省中医药管理局科技项目(2017153)
吉林省省级医药健康产业发展专项资金项目(20170311041YY)
吉林省教育厅"十三五"科学规划项目(JJKH20190470KJ)
长春中医药大学培育基金项目
