摘要
目的探讨Toll样受体4(TLR4)特异性抑制剂TAK-242对人多发性骨髓瘤细胞株RPMI8226增殖及凋亡的影响。方法取对数期生长的人多发性骨髓瘤细胞株RPMI8226分为A、B、C组及对照组(Control),A、B、C组分别加入终浓度为20、40、80μmol/L的TAK-242,对照组不加抑制剂,培养24 h后,采用CCK-8法检测细胞增殖抑制率;Annexin-V/PI检测细胞凋亡率;RT-PCR检测细胞TLR4、Myd88 mRNA的表达水平;Western blot检测细胞Myd88、NF-κB的蛋白表达情况。结果 A、B、C组细胞增殖抑制率、凋亡率升高,TLR4、Myd88mRNA表达水平及Myd88、NF-κB的蛋白表达水平降低,各浓度组之间的差异均有统计学意义(P<0.05)。结论 TAK-242可抑制人多发性骨髓瘤细胞RPMI8226的增殖,促进细胞凋亡,其机制可能为NF-κB信号通路功能被抑制。
Objective To investigate the effect of Toll-like receptor 4(TLR4) specific inhibitor TAK-242 on the proliferation and apoptosis of human multiple myeloma cell line RPMI8226. Methods Treated with 0,20, 40 and 80 μmol/L TAK-242, the human multiple myeloma cell line RPMI8226 with logarithmic growth were divided into control group, group A, group B and group C, respectively. After 24 hours of culture, the cell proliferation inhibition rate was detected by CCK-8 method;the apoptosis rate was detected by Annexin-V/PI;the expression levels of TLR4 and Myd88 mRNA were detected by RT-PCR;the protein expression levels of Myd88 and NF-κB were detected by Western blot. Results Compared with the control group, the cell proliferation inhibition rate and apoptosis rate of group A, B and C were increased;the expression levels of TLR4, Myd88 mRNA and the protein expression levels of Myd88 and NF-κB were decreased;and the differences among group A, B and C were statistically significant(P<0.05). Conclusion TAK-242 could inhibit the proliferation and promote the apoptosis of human multiple myeloma cell line RPMI8226, which may be related with the inhibition of NF-κB signaling pathway function.
作者
段萍萍
艾丽梅
DUAN Pingping;AI Limei(Department of Hematology,The First Hospital Affiliated to Jinzhou Medical University,Jinzhou 121000,China)
出处
《肿瘤防治研究》
CAS
CSCD
2019年第4期322-326,共5页
Cancer Research on Prevention and Treatment
基金
辽宁省自然科学基金指导计划(20180550001)
