摘要
目的:分析河南地区儿童急性淋巴细胞白血病(ALL)的常见白血病融合基因类型。方法:回顾性分析248例河南地区初诊ALL患儿的资料,应用多重巢式RT-PCR技术检测常见的15种ALL融合基因。结果:患儿男女比例为1. 38∶1,各年龄组患儿性别分布差异均无统计学意义(P=0. 568)。白血病基因阳性率39. 5%(98/248),E2A/PBX1阳性19 (7. 7%)例,BCR/ABL阳性22 (8. 9%)例,ETV6/RUNX1阳性26 (10. 5%)例,MLL阳性18(7. 3%)例,SIL/TAL1阳性8(3. 2%)例。不同年龄组白血病融合基因阳性率差异有统计学意义(P <0. 05),<1岁组MLL阳性率(20. 0%)最高,1～10岁组ETV6/RUNX1阳性率(14. 3%)最高;> 10岁组BCR/ABL阳性率(16. 9%)最高。结论:河南地区儿童ALL的常见突变基因类型与文献报道基本相符。
Aim:To analyze the leukemia fusion genotype in 248 children with acute lymphocytic leukemia(ALL)in Henan Province.Methods:Data of 248 newly diagnosed children with ALL in Henan Province were collected and analyzed,and 15 fusion genes commonly found in ALL were detected by multiple nested RT-PCR.Results:The ratio of male to female was 1.38∶1.There was no significant difference in the gender distribution among 3 groups based on age(P=0.568).The positive rate of leukemia fusion gene was 39.5%(98/248).Among them,26(10.5%)cases was ETV6/RUNX1 positive,22(8.9%)cases was BCR/ABL positive,19(7.7%)cases was E2A/PBX1 positive,18(7.3%)cases was MLL positive,and 8(3.2%)cases was SIL/TAL1 positive.The positive rate of leukemia fusion gene was different among 3 groups based on age(P<0.05).The positive rate of MLL gene in the<1 year group was the highest(20.0%),ETV6/RUNX1 gene positive rate in the 1-10 years group was the highest(14.3%),and BCR/ABL gene positive rate in the>10 years group was the highest(16.9%).Conclusion:The common mutated gene types of children leukemia in Henan Province are basically consistent with the literature reports.
作者
徐一卓
王西阁
陈力溶
XU Yizhuo;WANG Xige;CHEN Lirong(Department of Hematology,the Third Affiliated Hospital,Zhengzhou University,Zhengzhou 450052)
出处
《郑州大学学报（医学版）》
CAS
北大核心
2019年第3期470-473,共4页
Journal of Zhengzhou University(Medical Sciences)
