摘要
目的探讨弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中程序性死亡受体-1(programmed death-1,PD-1)及程序性死亡配体-1(programmed death-ligand 1,PD-L1)的表达及临床意义。方法采用免疫组化法检测184例DLBCL组织中PD-1、PD-L1的表达,分析两者表达与DLBCL临床病理特征的关系。结果 184例DLBCL中肿瘤细胞PD-1、PD-L1阳性率分别为1. 63%、43. 48%;微环境细胞PD-1、PD-L1阳性率分别为11. 41%、26. 09%。肿瘤细胞及微环境细胞PD-1的表达与患者性别、年龄、Hans分型、CD5、EBER、ALK以及CD30的表达差异均无显著性(P均> 0. 05)。非生发中心B细胞样(nongerminal center B-cell-like,non-GCB)型DLBCL肿瘤细胞(47. 55%)及微环境细胞(31. 47%)中PD-L1的阳性率高于生发中心B细胞样(germinal center B-cell-like,GCB)型DLBCL肿瘤细胞(29. 27%)及微环境细胞(7. 32%)(P均<0. 05); EBV+DLBCL肿瘤细胞(75. 00%)及微环境细胞(58. 33%)中PD-L1的阳性率高于EBV-DLBCL肿瘤细胞(40. 74%)及微环境细胞(24. 07%)(P均<0. 05); CD30+DLBCL(66. 67%)微环境细胞中PD-L1的阳性率高于CD30-DLBCL微环境细胞(27. 00%)(P=0. 005)。结论 PD-L1在non-GCB型以及EBV+DLBCL肿瘤细胞及微环境细胞中有更高的阳性率;且PD-L1在CD30+DLBCL微环境细胞中有更高的阳性率。
Purpose To evaluate programmed death-1 (PD-1) and programmed death-ligand 1 (PD-L1) expression in diffuse large B-cell lymphoma (DLBCL) and its relationship with clinicopathological characteristics. Methods Expression of PD-1 and PD-L1 was studied by immunohistochemical staining in 184 DLBCL tissues. Correlation of clinicopathological features with PD-1 and PD-L1 was analyzed in DLBCL. Results Immunohistochemical staining showed that 1.63% and 43.48% of 184 DLBCL samples expressed PD-1 and PD-L1 on tumor cells,respectively. While 11.41% and 26.09% of DLBCL samples expressed PD-1 and PD-L1 on microenvironment cells,respectively. PD-1 expression on both tumor cells and microenvironment cells was not correlated with gender,age,Hans algorithm,CD5,EBER,ALK,and CD30 expression (all P >0.05). PD-L1 expression rate on both tumor cells (47.55% vs 29.27%) and microenvironment cells (31.47% vs 7.32%) was significantly higher in non-germinal center B-cell-like (non-GCB) DLBCL subtypes compared to the germinal center B-cell (GCB) DLBCL subtypes (all P <0.05). Furthermore,PD-L1 expression rate on both tumor cells (75.00% vs 40.74%) and microenvironment cells (58.33% vs 24.07%) was significantly higher in EBV^+ DLBCL subtypes compared to the EBV^- DLBCL subtypes (all P <0.05). Moreover,PD-L1 expression rate on microenvironment cells was significantly higher in CD30^+ DLBCL subtypes (66.67%) compared to the CD30^- DLBCL subtypes (27.00%)( P =0.005). Conclusion PD-L1 expression rate on both tumor cells and microenvironment cells is significantly higher in non-GCB and EBV^+ DLBCL subtypes. Moreover,PD-L1 expression rate on microenvironment cells is significantly higher in CD30^+ DLBCL subtypes.
作者
尹海兵
吴雅珣
蔡南南
黄洁玉
王佳泰
代魁
何松
YIN Hai-bing;WU Ya-xun;CAI Nan-nan;HUANG Jie-yu;WANG Jia-tai;DAI Kui;HE Song(Department of Pathology,Nantong Tumor Hospital of Jiangsu Province,Nantong 226361,China)
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2019年第4期379-382,共4页
Chinese Journal of Clinical and Experimental Pathology
基金
国家自然科学基金面上项目(81570187)
南通市科技项目(HS2015003)
