摘要
目的 分析2016—2018年北京市西城区感染性腹泻病例中检出的肠炎沙门菌的耐药情况和分子分型特征。方法 对从腹泻患者便标本分离的39株沙门菌进行血清分型,采用微量肉汤法和脉冲场凝胶电泳(PFGE),对24株肠炎沙门菌进行药敏试验和分子分型。结果 肠炎沙门菌是北京市西城区优势菌型,24株肠炎沙门菌对阿奇霉素、头孢他啶、头孢噻肟、头孢西丁、氯霉素、亚胺培南和庆大霉素这7种抗生素均敏感;对萘啶酸、氨苄西林和氨苄西林/舒巴坦的耐药率较高;17株菌出现多重耐药。24株菌经XbaⅠ酶切分为12个带型和5个簇,33.33%菌株聚集在II簇,聚类相似性为96.00%,并且2个优势带型聚集在这一簇。结论 2016—2018年北京市西城区感染性腹泻病例中肠炎沙门菌耐药状况比较严重,需加强对多重耐药株的监控,肠炎沙门菌PFGE分子分型条带多样化,但存在一定聚集性。
[Objective]To analyze the antibiotic resistance and molecular typing characteristics of Salmonella enteritidis isolated from infectious diarrhea patients in Xicheng District of Beijing from 2016-2018.[Methods] 39 strains of Salmonella isolated from stool specimen of patients with diarrhea were identified by serotyping. Micro-dilution method and the pulsed-field gel electrophoresis(PFGE) were used to conduct the drug sensitivity test and molecular typing in 24 strain s of Salmonella enteritidis.[Results]Salmonella enteritidis was the predominant serotype in Xicheng District of Beijing. 24 strains of Salmonella enteritidis were sensitive to azithromycin,ceftazidine,cefotaxime,cefoxitin,chloromycetin,imipenem and gentamicin,but showed high resistance to nalidixic acid,ampicillin and ampicillin/sulbactam. Multidrug resistant was observed in 17 strains. 24 strains were divided into 12 types of PFGE and 5 clusters by XbaⅠ,33.33% strains clustered in clade Ⅱ,with similarity of 96.00%,and two main PFGE pattern were in this cluster.[Conclusion] Salmonella enteritidis isolated from infectious diarrhea patients in Xicheng District of Beijing from 2016-2018 shows severe drug resistance. The surveillance for multidrug resistance still needs to be strengthened. PFGE patterns show diversity and have certain aggregation relationship.
作者
霍哲
王永全
徐俊
苗芳
崔京辉
HUO Zhe;WANG Yong-quan;XU Jun;MIAO Fang;CUI Jing-hui(Microbiological Lalboratory ,Xicheng Center of Disease Control and Prevention , Beijing, 100053 , China)
出处
《职业与健康》
CAS
2019年第8期1055-1058,共4页
Occupation and Health
关键词
肠炎沙门菌
脉冲场凝胶电泳
耐药性
分子分型
Salmonella enteritidis
Pulsed-field gel electrophoresis
Antibiotic resistance
Molecular typing
