摘要
目的:研究杨梅多酚提取物(CBE)对高糖损伤的INS-1细胞活力、凋亡和坏死以及Pdx-1蛋白表达水平的影响。方法:通过四甲基偶氮唑盐(MTT)法检测胰岛细胞活性,采用Hoechst 33342/PI双染法观察细胞的凋亡和坏死,同时以Western blot法检测Pdx-1蛋白的表达水平。结果:杨梅多酚提取物(0.5～20μg/mL)对高糖损伤INS-1细胞具有保护作用,其中5μg/mL CBE的保护效果较好,与损伤组相比,细胞活性上升14%以上。且在低质量浓度范围(0.5～5μg/mL),CBE的保护效果呈剂量效应关系。荧光染色结果表明杨梅多酚提取物对高糖诱导损伤的INS-1细胞凋亡具有明显的抑制作用。免疫印迹试验显示杨梅多酚提取物干预可改善INS-1细胞因高糖诱导的Pdx-1表达水平下调。结论:杨梅多酚提取物对高糖诱导损伤的INS-1细胞具有显著的保护作用,其机制可能与改善葡萄糖刺激的胰岛素分泌功能有关。
Objective: To study the effects of Chinese bayberry polyphenol extract(CBE) on the viability, apoptosis and Pdx-1 expression of INS-1 cells injured by supra-physiologic glucose(HG). Methods: The viability of islet β cell was measured with MTT(3-4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide), the apoptosis and necrocytosis of INS-1 cells were observed by 33342/pi Hoechst double staining, the expression of Pdx-1 was measured with Western blot. Results: Compared with HG group, CBE(0.5-20 μg/mL) exhibited protective effects on glucose induced glucotoxicity, with an increase up to 14% of INS-1 proliferation rate at the best mass concentration 5 μg/mL. And CBE significantly enhanced INS-1 cell viability in a dose-effect manner at low concentration(0.5-5 μg/mL). The fluorescent staining results indicated that the CBE exhibited potent anti-apoptotic effect on INS-1 cells induced by HG. Furthermore, immunoblotting analysis showed that treatment with CBE could up-regulated the expression of Pdx-1, which was down-regulated resulted from chronic HG explosure. Conclusion: Chinese bayberry polyphenol extract had a significant protective effect on HG-induced INS-1 injury, and its mechanism may be involved in the improvement of insulin secretion function.
作者
戚向阳
孟珂
章棋
刘合生
柳云丽
喻柯柯
陈秋平
曹少谦
Qi Xiangyang;Meng Ke;Zhang Qi;Liu Hesheng;Liu Yunli;Yu Keke;Chen Qiuping;Cao Shaoqian(College of Biological and Environmental Sciences,Zhejiang Wanli University,Ningbo 315100,Zhejiang)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2019年第4期72-78,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金项目(31171780)
关键词
杨梅多酚提取物
高糖损伤
INS-1细胞
保护作用
Chinese bayberry polyphenol extracts
damage induced by high glucose
INS-1 cells
protective effect
