摘要
【目的】探究高强度持续训练负荷对大鼠骨骼肌抗氧化功能的各项指标:总抗氧化能力(total antioxidant capacity,TAOC)、羟自由基(hydroxyl free radical,·OH)抑制能力、氧化产物丙二醛(malonaldehyde,MDA)、过氧化氢酶(catalase,CAT)及谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性的影响。【方法】采用SD大鼠跑台运动模型,进行高强度持续训练,按照训练时间分为2、4、6周组,并设立正常对照组。各组大鼠训练结束后用匀浆法制备骨骼肌匀浆介质,分光光度法测定骨骼肌T-AOC、·OH抑制能力、MDA、CAT以及GSH-Px活性。【结果】经过高强度持续训练,T-AOC显著高于正常对照组(P<0.05)。随训练时间的增加,大鼠骨骼肌T-AOC逐渐下降。经过训练后,·OH抑制能力相比正常对照组显著降低(P<0.05)。4周组和6周组·OH抑制能力相比2周组有所上升(P<0.05)。训练6周后·OH抑制能力与训练4周时相比无显著性差异。训练2周和6周后,MDA含量较对照组和训练4周组均显著升高(P<0.05)。训练2周、4周和6周时,相比对照组,大鼠骨骼肌CAT水平均显著升高(P<0.05)。且训练6周组CAT水平较训练2周和训练4周组也有显著升高(P<0.05)。训练4周和6周后,相比2周组,GSH-Px水平显著降低(P<0.05)。训练6周,GSH-Px和正常对照组相比无显著差异。【结论】训练初期高强度持续训练对骨骼肌抗氧化能力有影响,随着训练时间的延长,抗氧化功能损伤加重,骨骼肌易产生疲劳,对生物体正常运动行为能力产生不利影响。
【Objective】To study the effects of high-intensity continuous exercise on the antioxidant function of rat skeletal muscle,including total antioxidant capacity(T-AOC), the inhibition ability of hydroxyl free radical(·OH), malonaldehyde(MDA), catalase(CAT) and glutathione peroxidase(GSH-Px).【Methods】Treadmill exercise methods for SD rats were used to establish high-intensity continuous exercise training models. According to the training time, the rats in the three groups were trained for 2, 4 or 6 weeks, and the normal control group was set up. Homogenate medium of rat skeletal muscle in each group was prepared by homogenate method at the end of training. The activities of T-AOC, inhibition ability of ·OH, MDA, CAT and GSH-Px in skeletal muscle were measured by spectrophotometry.【Results】After high-intensity continuous exercise, T-AOC level was higher than that in the control group(P<0.05). And T-AOC level showed a decrease tendency with the extension of training time. After exercise, the inhibition ability of·OH was lower than that in the control group(P< 0.05). The inhibition ability of·OH in the 4-week and 6-week groups was higher than that in the 2-week group(P< 0.05). After high-intensity continuous exercise, the inhibition ability of·OH showed no significant difference between 4-week group and 6-week group. The MDA levels in the 2-week and 6-week groups were higher than those in the control group and 4-week group(P< 0.05). The CAT levels in the 2-week, 4-week and 6-week were higher than that in the control group(P< 0.05). And the CAT level in the 6-week group was higher than those in the 2-week group and 4-week group(P< 0.05). After 4-week and 6-week training, the level of GSH-Px was lower than that in the 2-week group(P< 0.05). The comparison on GSH-Px showed no significant difference between 6-week group and control group【.Conclusion】In the initial stage of exercise, high-intensity continuous exercise has an effect on the antioxidant capacity of skeletal muscle. With the prolongation of training time, the antioxidant function is seriously damaged, and the skeletal muscle is prone to fatigue, which has an adverse effect on the normal motor behavior of the organism.
作者
陈立军
任鹤菲
张馨予
张敏
CHEN Li-jun;REN He-fei;ZHANG Xin-yu;ZHANG Min(Department of Human Functional Physiology,Logistic University of PAP,Tianjin 300309,China)
出处
《武警后勤学院学报（医学版）》
CAS
2019年第1期10-13,共4页
Journal of Logistics University of PAP（Medical Sciences）
关键词
大鼠
骨骼肌
高强度持续训练
抗氧化功能
Rat
Skeletal muscle
High-intensity continuous exercise training
Antioxidant capacity
