摘要
目的探讨红景天提取物对糖尿病性骨质疏松大鼠牙槽骨丢失的保护作用。方法腹腔注射链脲佐菌素60 mg·kg^(-1)建立糖尿病大鼠模型,建模成功后,将动物分为空白组、模型组、红景天组,灌胃给予红景天提取物150 mg·kg^(-1)8周后,检测各组大鼠血糖、血清ALP、Ca、OPG、RANKL的水平,并计算OPG/RANKL比值;同时进行牙槽骨组织病理分析及破骨细胞计数。结果给予红景天后,大鼠血糖、血清ALP和RANKL较模型组明显降低(P <0. 01);大鼠血清Ca、OPG水平及OPG/RANKL比值较模型组明显升高(P <0. 05);组织学检查显示,模型组大鼠牙槽骨小梁稀疏、断裂,骨吸收严重,给予红景天后牙槽骨结构恢复完整;破骨细胞计数显示,模型组破骨细胞数量明显增加,给予红景天后牙槽骨破骨细胞数量减少。结论红景天提取物对糖尿病大鼠早期的牙槽骨丢失具有保护作用,其作用机制主要是调节ALP和OPG/RANKL/RANK系统,进而促进骨形成,抑制骨吸收,使骨转换趋于平衡。
Aim To investigate the protective effect of Rhodiola rosea extract on alveolar bone injury in diabetic osteoporosis rats. Methods A diabetic rat model was established by intraperitoneal injection of streptozotocin 60 mg·kg^-1 . After successful establishment of the model, the animals were divided into control group, model group and Rhodiola rosea group. Rhodiola rosea extract was administered orally for eight weeks at a dose of 150 mg·kg^-1 . The levels of blood glucose, serum ALP, Ca, OPG and RANKL were measured, and the ratio of OPG to RANKL was calculated. At the same time, the alveolar bone histopathology was analyzed, and the number of osteoclasts were counted. Results After treated with Rhodiola rosea extract for eight weeks, the blood glucose, the levels of serum ALP and RANKL in Rhodiola rosea group were significantly lower than those in model group( P <0.01). The serum levels of Ca, OPG and the ratio of OPG/RANKL significantly increased in Rhodiola rosea group compared with those of model group( P <0.05). Histological examination showed that alveolar bone trabeculae in model group were sparse and fractured, bone resorption was serious, and alveolar bone structure was restored after Rhodiola rosea extract administration. The number of osteoclasts in model group increased significantly, and the number of alveolar osteoclasts decreased after Rhodiola rosea extract administration. Conclusions Rhodiola rosea extract has protective effect on early alveolar bone injury in diabetic rats, and its mechanism is mainly by regulating ALP and OPG/RANKL/RANK system, and accordingly promoting bone formation and inhibiting bone resorption, and balancing bone turnover.
作者
郑红星
黄洁茹
祁珊珊
陈琛
何佳
赵婷
浦井非
赵曼
ZHENG Hong-xing;HUANG Jie-ru;QI Shan-shan;CHEN Chen;HE Jia;Zhao Ting;Pu Jing-fei;Zhao Man(College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi 723000, China;Chinese-German Joint Lab for Natural Product Research, Shaanxi University of Technology, Hanzhong, Shaanxi 723000, China;Vitamin D Research Institute, Shaanxi University of Technology, Hanzhong, Shaanxi 723000, China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2019年第6期850-853,共4页
Chinese Pharmacological Bulletin
基金
陕西理工大学博士后基金(No SLGBH16-03)
国家高端外国专家项目(No GDT20176100048)
关键词
红景天
糖尿病
牙槽骨
破骨细胞
骨质疏松
骨转换
Rhodiola rosea
diabetes
alveolar bone
osteoclasts
osteoporosis
bone turnover
