摘要
纤维素内切酶在应用中存在产量较低、热稳定性差和活性偏低等问题。纤维素内切酶产生菌的选育,异源表达和分子改造是解决这些问题的有效途径。产生菌的选育包括传统的选育和基于组学技术的筛选,后者更有效率。异源表达系统主要为大肠杆菌和毕赤酵母,而后者具有更多的优势。提高热稳定性和活性是分子改造的主要目标。改造酶的结构,可以提高热稳定性,而活性通常也能随之提高。理性化设计比传统分子改造技术具有更高的效率,是分子改造发展的主流技术。
The characterizations of endoglucanase such as lower yield, poor thermostability and lower activity have been perceived as major problems to application. The producing strains breeding, heterologous expression and molecular engineering of endoglucanase are efficient approaches to overcome the problems. To breed producing strains, traditional breeding and screening based on omics technology were used, and the latter is more effective. Escherichia coli and Pichia pastoris are major heterologous expression systems, and P. pastoris shows more advantages. Molecular engineering mainly focuses on improving thermostability and specific activity. Generally, the activity of enzymes could be enhanced when their thermostability were improved by modification of structure. Compared to the traditional technologies of molecular engineering, rational engineering exhibits higher efficiency and is the mainstream technology for development.
作者
林俊涵
潘志斌
林谢榕
柯轶
王娜
林晨
Lin Junhan;Pan Zhibin;Lin Xierong;Ke Yi;Wang Na;Lin Chen(Department of Food and Bioengineering, Fujian Vocational College of Bioengineering, Fuzhou 350002;Fuzhou Taijiang Ding Dong Rehabilitation Center, Fuzhou 350002, China)
出处
《广东化工》
CAS
2019年第10期103-105,共3页
Guangdong Chemical Industry
基金
高活性纤维素内切酶EGI工程菌的构建(No.JA15767)
纤维素酶法改性竹笋膳食纤维工艺的研究(kj201501)
关键词
纤维素内切酶
异源表达
分子改造
理性化设计
endoglucanase
heterologous expression
molecular engineering
rational engineering
