摘要
目的:研究白花蛇舌草提取物对卵巢癌大鼠的治疗效果及对大鼠雌二醇(E2)、孕酮(P)和促黄体生成素(LH)水平的影响。方法:选取健康SD大鼠30只,分为模型组、实验组和空白组,并对模型组和实验组大鼠进行卵巢癌模型建造,空白组不做处理。建模成功后给予实验组大鼠白花蛇舌草提取物注射治疗,对模型组和空白组大鼠注射等体积生理盐水干预,14d后将所有大鼠断头处死,采集标本,HE染色后观察病理学组织图,并对三组大鼠的肿瘤重量平均值、腹水量平均值、肿瘤体积以及抑瘤率进行测量计算。采用化学发光法检测三组大鼠性激素水平,Western blot检测p38、NF kBp65蛋白相对表达,酶联免疫吸附测定法检测炎症指标TNF-α和IL-2水平。结果:实验组大鼠的肿瘤重量平均值、腹水量平均值以及肿瘤体积均显著低于模型组大鼠,抑瘤率明显高于模型组大鼠(P<0.05)。实验组大鼠性激素E2、P表达水平明显低于模型组大鼠,LH表达水平明显高于模型组大鼠(P<0.05)。实验组大鼠p38、NF-kBp65相对蛋白表达量相比模型组均显著降低,同时实验组大鼠TNF-α和IL-2水平相比模型组大鼠也明显下调(P<0.05)。结论:白花蛇舌草提取物能够改善卵巢癌大鼠的性激素水平,通过对p38/NF-kBp65信号通路来使机体内的炎症指标下降,从而抑制肿瘤的发展,为临床中卵巢癌的治疗和预后提供理论依据。
Objective:To study the effect of hedyotis diffusa extract on estradiol (E2),progesterone (P) and luteinizing hormone (LH) levels in ovarian cancer rats.Methods:30 healthy SD rats were divided into model group,experimental group and blank group.The ovarian cancer model was established in model group and experimental group,and the blank group was not treated.After successful modeling,the rats in the experimental group were injected with the extract of Hedyotis diffusa,and the rats in the model group and the blank group were injected with the same volume of normal saline.After 14 days,all the rats were decapitated,and the specimens were collected.After HE staining,the pathological histogram was observed,and the average weight of the tumor and the average ascites volume of the three groups of rats were measured.The tumor volume and tumor inhibition rate were measured and calculated.Chemiluminescence was used to detect the levels of sex hormones,western blot to detect the relative expression of p38 and NF-kBp65 protein,and enzyme-linked immunosorbent assay to detect the levels of inflammatory markers TNF-a and IL-2.Results:The average weight,ascites and tumor volume of the experimental group were significantly lower than those of the model group,and the tumor inhibition rate was significantly higher than that of the model group (P<0.05).The relative protein expression of p38 and NF-kBp65 in the experimental group was significantly lower than that in the model group,and the levels of TNF-a and IL-2 in the experimental group were also significantly lower than those in the model group (P<0.05).Conclusion:The extract of Hedyotis diffusa can improve the sex hormone level of ovarian cancer rats,through the p38 / NF-kBp65 signaling pathway to reduce inflammation index in the body,thus inhibiting the development of tumor,and provide an important theoretical basis for clinical treatment and prognosis of ovarian cancer.
作者
常丰葛
张珂
马玉瑶
孙灵霞
葛倩倩
CHANG Fengge;ZHANG Ke;MA Yuyao(Department of Gynecology,Xuchang People Undefineds Hospital in Henan Province,Xuchang 461000)
出处
《陕西中医》
2019年第6期687-691,共5页
Shaanxi Journal of Traditional Chinese Medicine
基金
河南省医学科技攻关计划项目(201403124)
关键词
白花蛇舌草
卵巢癌
雌二醇
促黄体生成素
实验动物
SD大鼠
Hedyotis diffusa
Ovarian cancer
Estradiol
Luteinizing hormone
Experimental animals
SD rats
