摘要
目的:观察针刺足三里穴配期门穴对慢性酒精性肝损伤大鼠模型的保护作用。方法:40只SD大鼠采用随机数字表法随机分为空白组(A)、模型组(B)、针刺组(C)、阳性药物组(D),每组10只。前16天为适应性酒灌胃,B、C、D组逐渐将酒的度数从43°升至52°。造模成功后,空白组不做任何处理,药物组灌水飞蓟宾生理盐水溶液(1mL/100g/d),针刺组给予相应治疗,治疗4周。末次针刺治疗后取血并取肝组织做病理学检查。测定大鼠血清中天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)。用ELISA法检测IV型胶原(IV-C)和羟脯氨酸(HYP)。Westernblot检测肝组织中TGF-β蛋白表达。结果:与模型组比较,针刺组肝组织病理损伤减轻,血清中ALT、AST水平显著降低(P<0.01),HYP、IV-C水平降低(P<0.05),肝组织中TGF-β蛋白表达降低(P<0.05)。结论:期门穴配足三里穴能够提升肝功能水平,抑制肝纤维化,并且可能通过调控TGF-β/Smads信号通路最终降低肝纤维化。
Objective: To observe the protective effect of needling Zusanli(ST36) and Qimen(LR14) in rats with chronic alcoholic liver injury. Methods: 40 SD rats were randomly divided into the blank group, the model group, the acupuncture group, and the positive control group, with 10 rats in each group. After 16 d adaptive gavage with alcohol, the model was established by gradually increasing the alcohol from 43 degrees to 52 degrees. After successful modeling, the blank group was given normal saline(1 mL/100 g/d), the positive control group was given Silybin saline solution(1 mL/100 g/d), and the acupuncture group was given corresponding treatment. The treatment course was four weeks. After the last acupuncture treatment, the abdominal aorta blood was taken for testing and liver tissue was collected for pathological examination. Serum levels of aspartate aminotransferase(AST) and alanine aminotransferase(ALT) were determined, and Type IV collagen(IV-C) and hydroxyproline(HYP) were detected by ELISA method. Western blot analysis was used to detect the protein expression of TGF-β in liver tissue. Results: Compared to the model group, the pathological injury of liver tissue was reduced, the serum levels of ALT and AST were significantly decreased( P <0.01), the levels of HYP and IV-C were decreased( P <0.05), and the protein expression of TGF-β in liver tissue was reduced in the acupuncture group( P <0.05). Conclusion: Acupoint association of LR14 and ST36 can improve liver function, inhibit liver fibrosis, and may ultimately reduce liver fibrosis by regulating TGF-β/Smads signaling pathway.
作者
姜阳
王朝辉
李丽
穆双双
徐维茵
杨秀璐
JIANG Yang;WANG Zhaohui;LI Li;MU Shuangshuang;XU Weiyin;YANG Xiulu(Changchun University of Chinese Medicine, Changchun 130117, China)
出处
《针灸临床杂志》
2019年第5期58-61,共4页
Journal of Clinical Acupuncture and Moxibustion
基金
国家自然科学基金资助课题,编号:81774393
关键词
合募配穴
针刺
酒精肝
保护作用
He-Mu acupoint association
Acupuncture
Alcoholic liver
Protection effect
