摘要
以‘Bean Saint esprit a oeil rouge’‘Bean White rice’‘97-5 架豆’为材料,以菜豆组培苗的子叶、茎、叶作为外植体,分别在添加不同浓度激素(2,4-D、6-BA、KT)配比的MS 培养基中进行培养,观察其愈伤组织诱导情况,并选取长势较好的愈伤组织进行凝集素水平测定。结果表明:3 个菜豆品种的子叶、茎、叶愈伤组织的适宜培养基均为MS + 3 mg/L 2,4-D + 0. 5 mg/L 6-BA + 0. 5 mg/L KT。‘Bean Saint esprit a oeil rouge’的子叶与叶愈伤组织中菜豆凝集素的凝集活力最高,为0. 27 HU。因此,使用‘Bean Saint esprit a oeil rouge’的子叶或叶为外植体,在MS + 3 mg/L 2,4-D + 0. 5 mg/L 6-BA + 0. 5 mg/L KT 中进行培养可提取较多的菜豆凝集素,为进一步研究基于菜豆凝集素的生物农药提供了试验基础与数据支持。
The ′Bean Saint esprit a oeil rouge′′Bean White rice′′ 97-5 racks′ of common bean cultivars were chosen as raw materials. After seed germination,the cotyledons,stems and leaves of the beans were chosen as explants,respectively,and to culture them in MS medium with different concentrations of hormone(2,4-D,6-BA,KT)to observe the induction of callus and determine lectin level in callus. The results showed that the suitable medium of cotyledon,stem and leaf callus in the three common bean cultivars were MS + 3 mg/L 2,4-D + 0. 5 mg/L 6-BA + 0. 5 mg/L KT. The agglutination activity of plant lectin in the cotyledons and leaves callus of ′Bean Saint esprit a oeil rouge′ was the highest,it was 0. 27 HU. In conclusion,the cotyledons or leaves of ′Bean Saint esprit a oeil rouge′ were selected in medium of MS + 3 mg/L 2,4-D + 0. 5 mg/L 6-BA + 0. 5 mg/L KT which could produce the largest amount of lectin,it will provide the experimental basis and data support for further research of artificial production of bean lectin.
作者
陈高
刘彤
万何平
陈禅友
CHEN Gao;LIU Tong;WAN Heping;CHEN Chanyou(Hubei Provine Engineering Research Center for Legume Plants,Jianghan University,Wuhan 430056,Hubei,China)
出处
《江汉大学学报（自然科学版）》
2019年第3期209-215,共7页
Journal of Jianghan University：Natural Science Edition
基金
湖北省食用豆类植物自然科技资源中心项目(2015BCE091)
湖北省技术创新专项(重大项目)(2017ABA147)
湖北省食用豆类植物自然科技资源中心开放基金项目(2017-DZY03)
