摘要
目的:探讨GeneXpert MTB/RIF PCR(GeneXpert)检测技术在基层实验室结核病特定人群中的应用效果。方法:回顾性分析笔者所在医院2016年1月-2019年1月共300例痰液标本,分别通过涂片法、液体分枝杆菌培养管培养法及GeneXpert MTB/RIF进行检测,分析GeneXpert检测技术在结核病特定人群中的应用效果。结果:300例痰标本中,涂片法检测显示阳性52例,占比17.33%,阴性248例,占比82.67%;液体培养法检测显示阳性80例,占比26.67%,阴性220例,占比73.33%。GeneXpert检测显示阳性81例,占比27.00%,阴性219例,占比73.00%。以液体培养法为标准,涂片法敏感度60.00%(48/80),一致率88.00%(264/300);GeneXpert敏感度91.25%(73/80),一致率95.00%(285/300)。两种检测方法敏感度比较,差异有统计学意义(X^2=21.191,P=0.000);一致率比较,差异有统计学意义(X^2=9.450,P=0.002)。结论:GeneXpert检测技术具有操作简单、快速、安全等优势,且敏感性、特异性较高的特点,可作为基层实验室早期检测结核病的重要方法之一,值得推广应用。
Objective:To explore the application effect of GeneXpert MTB/RIF PCR in tuberculosis specific population in primary laboratory.Method:300 sputum specimens from January 2016 to January 2019 in our hospital were retrospectively analyzed.The application effect of GeneXpert in tuberculosis specific population was analyzed by smear method,liquid mycobacterium culture tube method and GeneXpert MTB/RIF.Result:Among 300 sputum specimens,52 were positive by smear method,accounting for 17.33%,248 were negative,accounting for 82.67%,80 were positive by liquid culture method,accounting for 26.67%,220 were negative,accounting for 73.33%.GeneXpert test showed 81 positive cases(27.00%) and 219 negative cases(73.00%).According to the standard of liquid culture method,the sensitivity of smear method was 60.00%(48/80),the consistency rate was 88.00%(264/300),the sensitivity of GeneXpert was 91.25%(73/80),and the consistency rate was 95.00%(285/300).The sensitivity of the two methods was significantly different( X^2=21.191,P=0.000)and the consistency rate was significantly different( X^2=9.450,P=0.002).Conclusion:GeneXpert detection technology has the advantages of simple operation,fast and safety,and has high sensitivity and specificity.It can be used as one of the important methods for early detection of tuberculosis in primary laboratories,and it is worth popularizing and applying.
作者
占启川
刘建成
陈清文
施素英
林玉发
陈云琴
林明良
赖繁彩
ZHAN Qichuan;LIU Jiancheng;CHEN Qingwen(Quangang District Hospital of Quanzhou City,Quanzhou 362801,China)
出处
《中外医学研究》
2019年第15期64-66,共3页
CHINESE AND FOREIGN MEDICAL RESEARCH
基金
福建省泉州市泉港区科技计划项目(项目编号:2018Z08)
