摘要
目的:探讨黄连解毒汤对KM小鼠T细胞组成及肠粘膜中细胞因子表达的影响。方法:用黄连解毒汤灌胃小鼠7d和14d后分别采集相关样本,ELISA法测定小鼠回盲段肠粘液中sIgA、IL-6及IL-22表达水平;流式细胞仪检测小鼠脾脏中CD3^+T、CD4^+T和CD8^+T含量。结果:与空白组相比,黄连解毒汤给药组CD3^+T含量明显升高,CD4^+T和CD8^+T含量则明显降低,灌胃7d与14d对于给药组CD3^+T、CD4^+T和CD8^+T含量无明显变化;黄连解毒汤给药组sIgA浓度明显升高,IL-6含量则明显降低,灌胃7d与14d对于给药组sIgA含量无明显变化,IL-6变化不明显,IL-22于7d升高而于14d降低,具有极显著差异(P<0.01)。结论:黄连解毒汤能促进CD3^+T和sIgA分泌,抑制CD4^+T、CD8^+T和IL-6分泌和表达,对IL-22的表达则具有先促进后抑制的作用。
Objective: To investigate the effect of Huanglian Jiedu Decoction on T cell composition and cytokine expression in intestinal mucosa of KM mice. Methods: The expression levels of sIgA,IL-6 and IL-22 in ileocecal intestinal mucus of mice were detected by Elisa with Huanglian Jiedu Decoction for 7 days and 14 days, respectively. Flow cytometry assay was used to detect the levels of CD3^+T CD4^+T and CD8^+T in mouse spleen. Results: Compared with the blank group, the content of CD3^+T in the Huanglian Jiedu Decoction group was obviously increased and the content of CD4^+T and CD8^+T decreased significantly. The content of CD3^+T, CD4^+T and CD8^+T in the administration group was not obviously changed after intragastric administration for 7 d and 14 d. The concentration of sIgA of the Huanglian Jiedu Decoction group increased obviously, the content of IL-6 was observed decreased. After 7 d and 14 d, the content of sIgA and IL-6 showed no significant change. The concentration of IL-22 increased after 7 d and decreased significantly after 14 d(P< 0.01). Conclusion: Huanglian Jiedu Decoction can promote the secretion of CD3^+T and sIgA, inhibit the secretion and expression of CD4^+T, CD8^+T and IL-6. For IL-22 expression,it was promoted first and inhibited later.
作者
李慧
刘茜明
杨光勇
何光志
王文佳
田维毅
蔡琨
王平
黄高
梁建东
陈瑞
LI Hui;LIU Qianming;YANG Guangyong;HE Guangzhi;WANG Wenjia;TIAN Weiyi;CAI Kun;WANG Ping;HUANG Gao;LIANG Jiandong;CHEN Rui(Guizhou University of Traditional Chinese Medicine,Guizhou,Guiyang,550002)
出处
《贵阳中医学院学报》
2019年第3期28-32,共5页
Journal of Guiyang University of Chinese Medicine
基金
贵州省教育厅创新群体重大研究项目,项目编号:黔教合KY字[2016]036
贵中医科院内项目,项目编号:[2016]31号
教育部科学技术研究重点项目,项目编号:212153
国家自然科学基金项目,项目编号:81360589
贵州省高等学校工程研究中心项目,项目编号:黔教合KY字20153377
贵州省国内一流建设学科项目,项目编号:(中药学)(GNYL)[2017]008号联合资助
关键词
黄连解毒汤
免疫细胞
小鼠
细胞因子
Huanglian Jiedu Decoction
immunocyte
mice
cell factor
