摘要
目的评价应用流式荧光技术联合检测多种肿瘤标志物在肿瘤的早期诊断的价值。方法使用Luminex200多功能流式点阵检测系统测定分析421例恶性肿瘤患者、372例良性疾病患者和154名门诊健康体检者血清7种肿瘤标志物:甲胎蛋白(AFP)、糖类抗原125(CA125)、CYFRA211、糖类抗原42(CA242)、癌胚抗原(CEA)、β-人绒毛膜促性腺激素(β-HCG)、神经元特异性烯醇化酶(NSE)。结果恶性肿瘤组、良性疾病组、健康体检组阳性率分别为73.63%、28.77%、7.23%。恶性肿瘤组阳性率显著高于良性疾病组和健康体检组(P<0.01);多种肿瘤标志物联合检测对肝癌组、肺癌组、结直肠癌阳性检出率均显著高于其单一肿瘤标志物检测(P<0.01)。结论使用Luminex200多功能流式点阵系统联合检测多种肿瘤标志物可以显著提高恶性肿瘤诊断的敏感性,对肿瘤的早期诊断具有重要的应用价值。
Objective To evaluation flow fluorescence techniques were used to detect the significance of multiple tumor markers for early diagnosis.Method Using Luminex200 Multifunction flow lattice detection system analysis of 421 patients with malignant tumor,372 cases of patients with benign disease and154 cases of outpatient health check-up 7 kinds of serum tumor markers:a fetoprotein(AFP),carbohydrate antigen 125(CA125),CYFRA211,carbohydrate antigen 42(CA242),carcinoembryonic antigen(CEA),beta human chorionic gonadotropin(beta HCG),neuron specific enolization enzyme(NSE).Results malignant tumor,benign disease group and health check-up group positive rate were 73.63%,28.77%and 7.23%.Malignant tumor group were significantly higher than that of benign disease group and health check-up group(P<0.01);cooperative detection of multiple tumor markers for liver cancer,lung cancer,colorectal cancer detection rates were significantly higher than the single tumor markers detection(P<0.01).Conclusion Using Luminex200 multi-functional flow lattice system combined detection of multiple tumor markers can significantly improve the sensitivity of the malignant tumor diagnosis,It has important application value in the early diagnosis of tumor.
作者
梁仲城
李春丽
周培刚
黄艳云
LIANG Zhong-cheng;LI Chun-li;ZHOU Pei-gang;HUANG Yan-yun(Department of Clinical Laboratory, Baise People's Hospital, Baise 533000, China)
出处
《中国医药指南》
2019年第14期131-133,共3页
Guide of China Medicine
关键词
流式荧光技术
多种联合检测
肿瘤标志物
Flow fluorescence technique
Multiple joint detection
Tumor markers
