摘要
目的建立超高效液相色谱-串联四级杆飞行时间质谱(UPLC-Q-TOF/MSE)快速筛查人体尿液中58种毒品及滥用药物的方法。方法样品经甲醇蛋白沉淀法进行前处理。目标物经ACQUITY UPLC HSS C18色谱柱分离,以0.1%甲酸-乙腈溶液及5mmol/L甲酸铵-水溶液(pH=3)为流动相进行梯度洗脱,质谱采用电喷雾电离正离子全扫描模式(MS^E)检测。利用UNIfi软件建立数据库,并对样品进行筛查分析。结果人体尿液中58种毒品及滥用药物的检出限(LOD,3S/N)在0.2~25.0ng/mL之间,用UNIFI软件对尿液样品中添加的58种毒品及滥用药物进行快速筛查,通过数据库比对,目标化合物均被检出。结论方法的准确性高,操作简单、快速,适用于尿液中毒品及滥用药物的快速筛查。
Objective To establish a method for rapid screening of 58 drugs in human urine by ultra-high performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MSE).Methods The spiked samples were pretreated by protein precipitation with methanol,thereby having the target compounds separated from Acquity UPLC HSS C18 column that was flowed through gradient elution of 0.1% formic acid-acetonitrile and 5mmol/L ammonium formate-water (pH=3) mixture,the mobile phase.The electrospray ionization in positive ion was used for analysis in full scanning mode (MSE).UNIfisoftware was selected to set up the database and to process samples.Results The limit of detection (LOD,3S/N) for 58 drugs in human urine was in the range of 0.2~25.0ng/ml.The target compounds were all detected by the established database from UNIfisoftware.Conclusions The method is accurate,simple and rapid,suitable for rapid screening of abused drugs in urine.
作者
张文竞
赵蒙
杨景卫
王朝虹
李虹
徐曼曼
ZHANG Wenjing;ZHAO Meng;YANG Jingwei;WANG Zhaohong;LI Hong;XU Manman(School of Forensic Medicine,Kunming Medical University,Kunming 650500,China;Procuratoral Technology and Information Research Center,Supreme People’s Procuratorate,Beijing 100144,China;Xishuangbanna Prefectural Public Security Bureau,Jinghong 666100,Yunnan,China;MPS(Ministry of Public Security)’ Key Laboratory of Narcotics Assay and Control Technology,Kunming 650228,China)
出处
《刑事技术》
2019年第3期219-223,共5页
Forensic Science and Technology
基金
公安部科技强警基础工作专项项目(No.2016GABJC50)
