摘要
以三氟氯氰菊酯为模板分子,基于电沉积壳聚糖制备能够特异识别模板分子及其结构类似物的分子印迹电化学传感器,探索电沉积电位、电沉积时间对印迹传感器性能的影响。实验采用循环伏安法(CV)和差分脉冲伏安法(DPV)研究了传感器的电化学响应特性,并优化制备和检测条件,最终选择在-1.5V的电位下电沉积5min制备三氟氯氰菊酯分子印迹电化学传感器,并将该传感器应用于农产品中三氟氯氰菊酯残留的快速分析。结果表明,在最佳条件下,三氟氯氰菊酯在0.1~1.0μmol/L浓度范围内有良好的线性关系,检出限(S/N=3)为0.04μmol/L,样品加标回收率在81.2%~94.6%之间(n=5),相对标准偏差(RSD)≤4.1%。该传感器操作简便、检测快速灵敏、成本低、抗干扰能力强、稳定性好、具有良好的应用前景。
In this research, a molecularly imprinted electrochemical sensor based on the preparation of electrodeposited chitosan adopted was capable of identifying template molecules and their structural analogues using cypermethrin as template molecule. The effect of electrodeposition potential and electrodeposition time on the sensor performance was investigated by electrochemical methods, and the preparation and detection conditions of the sensor were optimized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Finally, cyhalothrin molecular imprinting electrochemicalsensor was prepared by electrodeposition at the potential of -1.5 V and electrodepositionfor 5 min, and the sensor was applied to rapid analysis of cyhalothrinresiduesin grain. The results showed that the sensor had a good selectivity under the optimal conditions, the linear relationship was found in the range of 0.1~ 1.0 μmol/L with the detection limit (S/N=3)of 0.04 μmol/L,the recoveries of the spiked samples were between 81.2% and 94.6%( n =5) and the relative standard deviation (RSD) was ≤4.1%. This sensor was simple, sensitive, with low cost, detection quickness, and strong anti-interference ability and good stability, presenting a good application prospect.
作者
赵玲钰
庞军
高文惠
Zhao Lingyu;Pang Jun;Gao Wenhui(College of Bioscience and Bioengineering,Hebei University of Science and Technology,Center for Fermentation Engineering of Hebei Province,Shijiazhuang 050000)
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2019年第5期134-139,146,共7页
Journal of the Chinese Cereals and Oils Association
基金
河北省科技计划项目(16275503D)
石家庄市科学技术研究与发展计划项目(181490372A)
关键词
三氟氯氰菊酯
电沉积
分子印迹聚合物
电化学传感器
快速分析
cyhalothrin
electrodeposition
molecular imprinting polymer
electrochemical sensor
rapid analysis
