摘要
试验旨在研究虾青素对脂多糖(LPS)诱导的小鼠急性结肠炎及氧化应激的影响。选择 6 周龄健康雄性 ICR 小鼠 32 只,随机分为 4 组,其中对照组和 LPS 组小鼠连续 15 d 灌胃橄榄油,虾青素组和虾青素保护组小鼠连续 15 d 灌胃 50 mg/kg 虾青素,15 d 后对照组和虾青素组腹腔注射生理盐水,LPS 组和虾青素保护组腹腔注射 1 mg/kg LPS,3 h 后处死,采集血液及结肠组织。ELISA 和荧光定量 PCR 检测血清及结肠中炎性因子和抗氧化酶水平,生物化学法测定结肠中氧化水平,Western blot 检测结肠中 p-NF-κB p65 蛋白相对表达量,HE 染色观察结肠病理形态学变化。结果表明:与对照组相比,LPS 组小鼠血清和结肠组织中炎症因子的表达及 NF-κB p65 蛋白的活化均显著升高(P<0.05),组织抗氧化水平低(P<0.05),结肠黏膜损伤严重;与 LPS 组相比,保护组小鼠血清和结肠组织中炎症因子表达及 NF-κB p65 蛋白的活化均显著降低(P<0.05),组织抗氧化水平高(P<0.05),且结肠黏膜损伤程度低。虾青素可保护小鼠结肠黏膜结构,通过抑制 NF-κB信号通路降低机体中炎性因子的分泌,提高组织抗氧化水平,缓解 LPS 引起的急性结肠损伤。
To investigate the effects of astaxanthin (ATX) on acute colon inflammation and oxidative stress induced by lipopolysaccharide (LPS) in mice. Thirty-two healthy male ICR mice were randomly allocated into four groups. ATX and olive oil was administrated by oral gavage for 15 d consecutively before the saline or LPS(1mg/kg) injected. Blood and colon tissues were collected after 3h later. The levels of in flammatory factors and antioxidant enzyme in serum or colon were measured by ELISA or fluorescence quantitative PCR. The activities of antioxidant enzyme were measured by biochemical methods. p-NF-κB p65 protein was measured by western blot. H&E staining was used to observe the histopathological changes. Compared with the control group, the expression of in flammatory factors and the activation of NF-κB p65 protein in serum and colon tissues of LPS group were signi ficantly increased (P<0.05), the tissue antioxidant level was low, and the colonic mucosa was severely damaged. Compared with LPS group, the expression of in flammatory factors and the activation of NF-κB p65 protein in serum and colon tissues of the protective group were significantly decreased (P<0.05), and the tissue antioxidant level was high. In addition, the degree of colon mucosal damage was low. ATX treatment can protect colonic mucosa structure in mice, and relieve acute colon injury by suppressing the secretion of in flammatory cytokines, increase antioxidant levels and inhibiting the activation of NF-κB p65 protein in NF-κB pathway in LPS-induced mice.
作者
张子琪
朱凌羽
刘惠麟
李若楠
郎伍营
吴旻
兰海楠
郑鑫
ZHANG Zi-qi;ZHU Ling-yu;LIU Hui-lin;LI Ruo-nan;LANG Wu-ying;WU Min;LAN Hai-nan;ZHENG Xin(College of Animal Science and Technology,Jilin Agricultural University,Jilin Changchun 130118,China)
出处
《中国畜牧杂志》
CAS
北大核心
2019年第6期107-112,共6页
Chinese Journal of Animal Science
基金
国家自然科学基金(31672511)
