摘要
背景与目的:CD44分子是众多肿瘤细胞的标志分子,其表达水平与肿瘤细胞的恶性程度有关。该研究探讨CD44基因中的单核苷酸多态性(single nucleotide polymorphism,SNP)位点与云南汉族人群宫颈癌和非小细胞肺癌(non-small cell lung cancer,NSCLC)易感性的相关性。方法:选取了CD44基因中的两个SNP位点rs13347和rs8193,采用TagMan基因分型的方法,分析这两个多态性位点在497例宫颈癌患者和500例健康对照个体以及483例NSCLC患者和471例健康对照个体中的分布特征,并分析CD44基因中的多态性位点与云南汉族人群宫颈癌和NSCLC的相关性。结果:rs13347和rs8193位点等位基因和基因型在宫颈癌组和对照组中的分布频率的差异无统计学意义(P>0.05)。而在NSCLC组和对照组的比较中:rs13347和rs8193位点等位基因在NSCLC组和对照组中的分布频率的差异有统计学意义(P=0.020和P=0.004);这两个位点基因型在NSCLC组和对照组中分布频率的差异有统计学意义(P=0.027和P=0.020);其中rs13347位点等位基因C在NSCLC组中的分布频率显著高于对照组,可能是NSCLC发生的风险因素(OR=1.250,95%CI:1.035~1.509),rs8193位点等位基因C在对照组中的分布频率显著高于NSCLC组,可能是NSCLC发生的保护性因素(OR=0.768,95%CI:0.641~0.921)。单倍型分析结果显示,rs13347C-rs8193T和rs13347T-rs8193C在NSCLC组和对照组中的分布频率差异有统计学意义(P=0.003和0.022);该结果说明单倍型rs13347C-rs8193T可能是云南汉族人群NSCLC发生的风险性因素(OR=1.316,95%CI:1.096~1.579)。结论:CD44基因中的两个SNP位点rs13347和rs8193可能与云南汉族人群宫颈癌发病风险无关,而可能与云南汉族人群NSCLC具有相关性。
Background and purpose: CD44 is a biomarker for many human malignant tumors, and the expression level of CD44 might be associated with malignancy of cancers. Thus, in the current study, we investigated the association between single nucleotide polymorphisms(SNP) in CD44 gene and cervical cancer and non-small cell lung cancer(NSCLC) in a Yunnan Han population.Methods: For cervical cancer, 497 patients and 500 healthy individuals were recruited. For NSCLC, 483 patients and 471 healthy individuals were recruited. Two SNP in CD44 gene(rs13347, rs8193) were genotyped using TaqMan assay. And the association between the two SNP and the tumors was analyzed. Results: For cervical cancer, the allelic and genotypic frequencies of rs13347 and rs8193 were not significantly different between patients with cervical cancer and control groups(P>0.05). For NSCLC, the allelic frequencies of rs13347 and rs8193 were significantly different between NSCLC and control groups(P=0.020 and P=0.004). The C allele of rs13347 occurred more frequently in NSCLC group than in control group and might be the risk factor for NSCLC(OR=1.250, 95% CI: 1.035-1.509). The C allele of rs8193 occurred significantly more frequently in control group compared with NSCLC group, and might be a protective factor for NSCLC(OR=0.768, 95% CI: 0.641-0.921). The genotypic frequencies of rs13347 and rs8193 were significantly different between NSCLC and control groups(P=0.027 and P=0.020). Additionally, the results of haplotype analysis showed that rs13347 C-rs8193 T haplotype might be associated with higher risk of NSCLC(P=0.003, OR=1.316, 95% CI: 1.096-1.579). Conclusion: The two SNP in the CD44 gene(rs13347, rs8193) might be only associated with the risk of NSCLC, but not cervical cancer in the Yunnan Han population.
作者
赵雨笛
谭芳
严志凌
李盈甫
戴书颖
周紫云
张新文
刘舒媛
史荔
姚宇峰
ZHAO Yudi;TAN Fang;YAN Zhiling;LI Yingfu;DAI Shuying;ZHOU Ziyun;ZHANG Xinwen;LIU Shuyuan;SHI Li;YAO Yufeng(Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Kunming 650118, Yunnan Province, China;Department of Geriatrics, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China;Department of Gynaecologic Oncology, the Third of Kunming Medical University, Kunming 650118, Yunnan Province, China;School of Basic Medical Science, Kunming Medical University, Kunming 650500, Yunnan Province, China;School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming 650500, Yunnan Province, China)
出处
《中国癌症杂志》
CAS
CSCD
北大核心
2019年第4期277-283,共7页
China Oncology
基金
国家自然科学基金(81573206)
云南省应用基础研究计划(2016FA034)
云南省应用基础研究计划-昆明医科大学联合专项[2017FE467(-077)、2017FE467(-012)、2017FE468(-193)、2018FE001(-254)和2018FE001(-219)]
中国医学科学院重大协同创新项目(2016-12M-2-001)
云南省卫生厅科技处资助项目(2017NS189)
关键词
宫颈癌
非小细胞肺癌
恶性肿瘤
CD44
单核苷酸多态性
遗传易感性
云南汉族人群
Cervical cancer
Non-small cell lung cancer
Malignant tumor
CD44
Single nucleotide polymorphisms
Genetic susceptibility
Yunnan Han population
