摘要
菌核生枝顶孢霉(Acremoniumsclerotigenum)作为枝顶孢属真菌的优势种,其次生代谢产物多样。为了明确其对植物病害致病菌的抑制效果,以桃褐斑致病菌(Alternariaalternata)为靶标菌,采用菌丝生长速率法和孢子萌发法测定6种发酵液的抑菌活性。结果表明:PD培养基发酵液提取物对桃褐斑致病菌的抑制效果最好,对菌丝生长和孢子萌发的EC50分别为53.6734mg/L和808.1584mg/L;培养5d的PD培养基发酵液的抑菌效果最好,对菌丝生长和孢子萌发的EC50分别为1.4458mg/L和309.6318mg/L;菌核生枝顶孢霉发酵液对桃褐斑致病菌菌丝生长的抑制效果优于对孢子萌发效果。该研究结果为将其开发成生物源农药奠定了理论基础。
Acremonium sclerotigenum, as the dominant species of Acremonium. sp, its secondary metabolites are diverse. In order to clarify its inhibitory effect on pathogenic bacteria, in this study, Alternaria alternata was used as the target bacteria, and the antibacterial activities of the six fermentation broths were determined by mycelial growth rate method and spore germination method. The results showed that: The fermentation broth extract of PD medium had the best inhibitory effect on A. alternata, and the EC50 of mycelial growth and spore germination respectively were 53.673 4 mg/L and 808.158 4 mg/L;Fermentation broth of PD culture medium cultured for 5 d has the best antibacterial effect, and the EC50 of mycelial growth and spore germination respectively were 1.445 8 mg/L 309.631 8 mg/L;The inhibitory effect of A. sclerotigenum. fermentation broth on mycelial growth is better than the spore germination. The study laid the theoretical foundation for the development of biogenic pesticides.
作者
姚秀英
刘南南
王桂清
YAO Xiuying;LIU Nannan;WANG Guiqing(Agricultural College,Liaocheng University,Liaocheng Shandong 252059,China)
出处
《农业科技与装备》
2019年第3期18-22,共5页
Agricultural Science & Technology and Equipment
基金
山东省重点研发计划项目(2014GNC110020)
山东省聊城市科技发展计划项目(2014GJH10)
2018年国家级大学生创新项目(201810447033)
关键词
菌核生枝顶孢霉
内生菌
次生代谢物
培养条件
抑菌活性
Acremonium sclerotigenum
endophyte
secondary metabolites
condition of culture
bacteriostatic activity
