摘要
目的 观察高速离心前后脂血样本中9项肝功能结果的差异,分析高速离心是否能消除脂血对肝功能项目检测的影响。方法 随机收集50例脂血标本,经常规离心后用日立008全自动生化分析仪检测9项肝功能项目:总蛋白(TP)、清蛋白(ALB)、天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)、γ-谷氨酰基转肽酶(GGT)、总胆汁酸(TBA)、总胆红素(TBIL)、直接胆红素(DBIL)。经高速离心后吸弃上层乳糜微粒取下层清亮血清再次检测以上项目。比较高速离心前后结果的差异。结果 50例脂血标本TP、ALB、AST、ALT、TBA、DBIL6项肝功能项目经高速离心后结果差异无统计学意义(P>0.01),而ALP、GGT、TBIL3项经高速离心处理后检测结果差异有统计学意义(P<0.01)。结论 高速离心可以消除脂血对部分肝功能检测项目的影响。
Objective To observe the differences of nine biochemical liver function tests between routine centrifugal and high speed centrifugal method,and to investigate whether the high speed centrifugal method can eliminate the interference of lipid blood on biochemical liver function tests. Methods The lipid blood samples of 50 patients were collected randomly.After routine centrifugal,serum total protein (TP),albumin (ALB),aspartate amino transferase (AST),alanine amino transferase (ALT),alkaline phosphatase (ALP),γ-glutamyl transpoptidase (GGT),total bile acid (TBA),total bilirubin (TBIL),direct bilirubin (DBIL) were measured by the HITACHI 008 automatic biochemical analyzer.Then after high speed centrifugal,clear serum were measured again while the upper lipid were removed.The results were compared between routine centrifugal and high speed centrifugal methods. Results There were no significant differences in the results of liver function items of TP,ALB,AST,ALT,TBA and DBIL in 50 lipid-blood samples after high-speed centrifugation ( P >0.05),while there were significant differences in ALP,GGT and TBIL after high-speed centrifugation ( P <0.05). Conclusion High speed centrifugal method can eliminate the the interference of lipid blood on some biochemical liver function tests.
作者
解春宝
罗江蓉
向娜娜
传良敏
XIE Chunbao;LUO Jiangrong;XIANG Nana;CHUAN Liangming(Department of Clinical Laboratory,Sichuan Academy of Medical Sciences/Sichuan Provincial People′s Hospital,Chengdu,Sichuan 610072,China;Department of Operation,Sichuan Academy of Medical Sciences/Sichuan Provincial People′s Hospital,Chengdu,Sichuan 610072,China;Department of Medical Laboratory,North Sichuan Medical College,Nanchong,Sichuan 637000,China)
出处
《国际检验医学杂志》
CAS
2019年第11期1311-1313,共3页
International Journal of Laboratory Medicine
基金
四川省卫生和计划生育委员会科研课题(16PJ470)
四川省医学科学院·四川省人民医院青年基金项目(2015QN03)
四川省医学科学院·四川省人民医院苗圃科研课题重点项目(2014MP05)
关键词
高速离心法
肝功能
脂血
干扰
high speed centrifugal method
liver function
lipid blood
interference
