摘要
众所周知,中药成分复杂,多指标质量评价是今后趋势之一。但是,指标成分的选择及其代表性研究,往往是个棘手的难题。该文探索蟾蜍毒液中多种蟾毒配基含量的内在联系,寻找更加合理的蟾酥质控指标。该研究随机选取全国17个产地的样本,建立7种蟾毒配基含量数据库;然后,采用Spearman分析和PCA方法研究成分间的相关性,再用逐步回归分析建立总含量预测模型。研究发现,华蟾酥毒基、蟾毒灵和远华蟾蜍精之和与所测蟾毒配基总含量存在着相关性。据此推测,以这3种成分作为蟾酥质控指标,与现有的华蟾酥毒基和酯蟾毒配基质控指标相比,更能反映蟾毒配基的整体含量。这一发现有助于蟾酥质量标准的修改。
As known,simultaneous determination of various chemical indicators is one of the future trends in quality control of traditional Chinese medicines because of the extremely complex chemical compositions. This project is to screen the quality markers that can accurately control the quality of the Bufonis Venenum by exploring the intrinsic correlation of components. In this study,venom of Bufo bufo gargarizans from 17 different sources were used as research samples,and the contents of 7 bufogenin were determined by HPLC-DAD. Then,the data obtained were analyzed by Spearman correlation analysis and principal component analysis( PCA). In addition,a stepwise regression analysis was used to establish a predictive model for the contents of the seven bufogenin components( independent variable) and the total contents of the bufogenin( dependent variable). The results indicated that there is a significant positive correlation between the contents of telocinobufagin and cinobufotalin,and there is a significant positive correlation between the contents of bufalin,cinobufagin and resibufogenin. In contrast,the contents of telocinobufagin and cinobufotalin are negatively correlated with the contents of bufalin,cinobufagin and resibufogenin. However,the correlation between gamabufotalin and bufotalin and other components are not obvious. Furthermore,further study found that there is a correlation between the sum of the contents of bufalin,cinobufagin and telocinobufagin and the total contents of the bufogenin. In fact,the application of bufalin,cinobufagin and telocinobufagin as the quality control indicators of the Bufonis Venenum can better reflect the quality characteristics of the Bufonis Venenum compared with the previous quality control indicators. The conclusions will provide a reference for the revision of the quality standards of the Bufonis Venenum.
作者
曹月婷
崔可可
吴纪恒
潘红烨
卢正玉
邵家峰
王龙虎
CAO Yue-ting;CUI Ke-ke;WU Ji-heng;PAN Hong-ye;LU Zheng-yu;SHAO Jia-feng;WANG Long-hu(College of Pharmaceutical Sciences,Zhejiang University,Hangzhou 310058,Chirui;Jiangsu Jingchan Biological Resources Development Co.,Ltd.,Xuyi 211700. China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2019年第9期1850-1856,共7页
China Journal of Chinese Materia Medica
基金
智能制造专项(SQ2018ZX091726)
